Strain Data Sheet
RBRC02399
Information1 | |
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| Image | |
| BRC No. | RBRC02399 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-PRAT4A<tm1Kmiy> |
| Former Common name | C57BL/6-PRAT4A<-/-> |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NCrSlc |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Kensuke Miyake, The University of Tokyo in 2000. |
| Strain description | C57BL/6-PRAT4A<tm1Kmiy>. Immune cells such as dendric cells (DCs) and macrophages express multiple Toll-like receptors (TLRs) which recognize a variety of pathogen products. A protein associated with TLR4 (PRAT4A) has been found to bind TLR4, and regulate the expression of TLR4 and the response to LPS. Using PRAT4A-deficient mice, there are defective cytokine production against bacteria and activation of Th1 responses. DCs, macrophages and B cells lacking PRAT4A have impaired responses to a variety of TLR ligands. The PRAT4A-deficient bone marrow chimera mice are resistant to lethal-dose LPS administration in the LPS-induced sepsis model to analyze in vivo immune response. This mutant mouse is useful for the study of PRAT4A in innate and adaptive immunity. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6CrSlc] |
| References | J. Exp. Med., 204, 2963-2976 (2007) 17998391 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cnpy3canopy 3 homolog (zebrafish)17Cnpy3<tm1Kmiy>targeted mutation 1, Kensuke Miyake1600025D17Rik, 2410050O22Rik, CAG4A, ERDA5, Tnrc5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)17neo; neomycin; |
Information2 | |
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| Donor DNA | mouse PRAT4A genomic DNA, mouse PGK promoter, E. coli neo |
| Research application | Immunology and Inflammation Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Exp. Med., 204, 2963-2976 (2007).Transfer of the BIOLOGICAL RESOURCE to the third party from the RECIPIENT will never be done without a prior permission of the DEPOSITOR. The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Kensuke Miyake (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Mouse of the Month Apr 2008 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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