Strain Data Sheet
RBRC02393
Information1 | |
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| Image |  |
| BRC No. | RBRC02393 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C.Cg-Aicda<tm1Hon>(N12) |
| Former Common name | BALB/c-Aicda<-/->(N12)mouse |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | BALB/cCrSlc |
| Appearance | albino [A/A b/b c/c] |
| Strain development | Developed by Dr. Masamichi Muramatsu at Kyoto University in 2000. A neomycin resistance cassette was transferred into TT2 ES cells to replace exon 2 and part of exon 3 of the Aicda gene. The Aid deficient mice were backcrossed to BALB/c. |
| Strain description | Aid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency causes a complete defect in class switching and shows a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cells is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene is a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also found that somatic hypermutation of an immunoglobulin variable region gene and gene conversion did not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell. |
| Colony maintenance | Homozyogte x HomozygoteHomozygous mutant mice are viable and fertile. |
| References | Cell, 102, 553-563 (2000). 11007474 Science, 298, 1424-1427 (2002). 12434060 |
Health Report | |
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| Examination Date / Room / Rack | 2023/02/27Room:4-BRack:A 2022/11/29Room:4-BRack:A 2022/08/29Room:4-BRack:A 2022/05/31Room:4-BRack:A 2022/03/07Room:4-BRack:A |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA [Update June, 4, 2015] |
| Research application | Cre/loxP system Immunology and Inflammation Research |
| Specific Term and Conditions | 1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science, 298, 1424-1427 (2002). 3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE. |
| Depositor | Tasuku Honjo (Kyoto University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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