RBRC02393

Information1

Image
BRC No.RBRC02393
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Aicda<tm1Hon>(N12)
Former Common nameBALB/c-Aicda<-/->(N12)mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainBALB/cCrSlc
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Masamichi Muramatsu at Kyoto University in 2000. A neomycin resistance cassette was transferred into TT2 ES cells to replace exon 2 and part of exon 3 of the Aicda gene. The Aid deficient mice were backcrossed to BALB/c.
Strain descriptionAid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency causes a complete defect in class switching and shows a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cells is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene is a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also found that somatic hypermutation of an immunoglobulin variable region gene and gene conversion did not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell.
Colony maintenanceHomozyogte x HomozygoteHomozygous mutant mice are viable and fertile.
ReferencesScience, 298, 1424-1427 (2002). 12434060
Cell, 102, 553-563 (2000). 11007474

Health Report

Examination Date / Room / Rack2009/06/08Room:3-8Rack:C
2009/04/07Room:3-8Rack:C
2009/04/06Room:3-8Rack:C
2009/02/09Room:3-8Rack:C
2008/12/08Room:3-8Rack:C
2008/10/06Room:3-8Rack:C
2008/08/04Room:3-8Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Aicdaactivation-induced cytidine deaminase6Aicda<tm1Hon>targeted mutation 1, Tasuku Honjo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Activation-induced cytidine deaminase (AID) genomic DNA [Update June, 4, 2015]
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science, 298, 1424-1427 (2002).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data