RBRC02392

Information1

Image
BRC No.RBRC02392
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Jdp2<tm2Kkyo>/Rbrc
Former Common nameB6.129P2-Jundm2<tm2>
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Yokoyama KK and Pan J, RIKEN BioResource Center. The E14 ES cells originated from 129P2/OlaHsd were used. The knock-out mice are under backcrossing to C57BL/6J strain.
Strain descriptionJDP2 coding region was disrupted.
Colony maintenanceHeterozygote x Wild-type [C57BL/6J]
References

Health Report

Examination Date / Room / Rack2013/07/22Room:4-ARack:I
2013/07/17Room:4-ARack:I
2013/07/16Room:4-ARack:I
2013/05/20Room:4-ARack:I
2013/03/25Room:4-ARack:I
2013/01/21Room:4-ARack:I
2012/11/26Room:4-ARack:I
2012/09/24Room:4-ARack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Jdp2Jun dimerization protein 212Jdp2<tm2Kkyo>targeted mutation 2, Kazunari K Yokoyama

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, S. cerevisiae Frt sites, mouse JDP2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER Dr. Kazushige YOKOYAMA (kazu@kmu.edu.tw).
DepositorYuichi Obata (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Yao C, Yao GQ, Sun BH, Zhang C, Tommasini SM, Insogna K.
The transcription factor T-box3 regulates Colony Stimulating Factor 1-dependent Jun Dimerization Protein 2 expression and plays an important role in osteoclastogenesis.
J. Biol. Chem. (2014) 24394418