Strain Data Sheet

RBRC02388

update : 2023-09-20

Information1
Image
BRC No.	RBRC02388
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129P2-Ly96<tm1Kmiy>
Former Common name	C57BL/6-MD-2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References	Nat. Immunol. 3, 667-672 (2002) 12055629

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ly96lymphocyte antigen 961Ly96<tm1Kmiy>targeted mutation 1, Kensuke MiyakeESOP-1, MD-2, MD2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2
Donor DNA	mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Ly96 genome DNA
Research application	Immunology and Inflammation Research
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Immunology, 3, 667-672 (2002).
Depositor	Kensuke Miyake (The University of Tokyo)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month)
Additional Info.	Genotyping protocol -PCR- Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Lab HP (Japanese)

BRC mice in Publications

Fang Q, Wang J, Zhang Y, Wang L, Li W, Han J, Huang W, Liang G, Wang Y. Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis. Biochim Biophys Acta Mol Basis Dis 1864(1) 252-262(2018) 28965884 Wang Y, Fang Q, Jin Y, Liu Z, Zou C, Yu W, Li W, Shan X, Chen R, Khan Z, Liang G. Blockade of myeloid differentiation 2 attenuates diabetic nephropathy by reducing activation of the renin-angiotensin system in mouse kidneys. Br J Pharmacol 176(14) 2642-2657(2019) 30959575 Wang Y, Shan X, Chen G, Jiang L, Wang Z, Fang Q, Liu X, Wang J, Zhang Y, Wu W, Liang G. MD-2 as the target of a novel small molecule, L6H21, in the attenuation of LPS-induced inflammatory response and sepsis. Br J Pharmacol 172(17) 4391-405(2015) 26076332 Luo W, Ye L, Hu XT, Wang MH, Wang MX, Jin LM, Xiao ZX, Qian JC, Wang Y, Zuo W, Huang LJ, Liang G. MD2 deficiency prevents high-fat diet-induced AMPK suppression and lipid accumulation through regulating TBK1 in non-alcoholic fatty liver disease. Clin Transl Med 12(3) e777(2022) 35343085 Rajamanickam V, Yan T, Xu S, Hui J, Xu X, Ren L, Liu Z, Liang G, Wang O, Wang Y. Selective targeting of the TLR4 co-receptor, MD2, prevents colon cancer growth and lung metastasis. Int J Biol Sci 16(8) 1288-1302(2020) 32210720 Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G. Inhibition of MD2-dependent inflammation attenuates the progression of non-alcoholic fatty liver disease. J Cell Mol Med 22(2) 936-947(2018) 29077272 Fang Q, Wang L, Yang D, Chen X, Shan X, Zhang Y, Lum H, Wang J, Zhong P, Liang G, Wang Y. Blockade of myeloid differentiation protein 2 prevents obesity-induced inflammation and nephropathy. J Cell Mol Med 21(12) 3776-3786(2017) 28767204 Wang Y, Luo W, Han J, Khan ZA, Fang Q, Jin Y, Chen X, Zhang Y, Wang M, Qian J, Huang W, Lum H, Wu G, Liang G. MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy. Nat Commun 11(1) 2148(2020) 32358497 Wang L, Han J, Shan P, You S, Chen X, Jin Y, Wang J, Huang W, Wang Y, Liang G. MD2 Blockage Protects Obesity-Induced Vascular Remodeling via Activating AMPK/Nrf2. Obesity (Silver Spring) 25(9) 1532-1539(2017) 28726347 Chen H, Zhang Y, Zhang W, Liu H, Sun C, Zhang B, Bai B, Wu D, Xiao Z, Lum H, Zhou J, Chen R, Liang G. Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury. Phytomedicine 63 152997(2019) 31254764 Chen H, Song Z, Ying S, Yang X, Wu W, Tan Q, Ju X, Wu W, Zhang X, Qu J, Wang Y. Myeloid differentiation protein 2 induced retinal ischemia reperfusion injury via upregulation of ROS through a TLR4-NOX4 pathway. Toxicol Lett 282 109-120(2018) 29111459

BRC mice in Publications

Fang Q, Wang J, Zhang Y, Wang L, Li W, Han J, Huang W, Liang G, Wang Y.
Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis.
Biochim Biophys Acta Mol Basis Dis 1864(1) 252-262(2018) 28965884

Wang Y, Fang Q, Jin Y, Liu Z, Zou C, Yu W, Li W, Shan X, Chen R, Khan Z, Liang G.
Blockade of myeloid differentiation 2 attenuates diabetic nephropathy by reducing activation of the renin-angiotensin system in mouse kidneys.
Br J Pharmacol 176(14) 2642-2657(2019) 30959575

Wang Y, Shan X, Chen G, Jiang L, Wang Z, Fang Q, Liu X, Wang J, Zhang Y, Wu W, Liang G.
MD-2 as the target of a novel small molecule, L6H21, in the attenuation of LPS-induced inflammatory response and sepsis.
Br J Pharmacol 172(17) 4391-405(2015) 26076332

Luo W, Ye L, Hu XT, Wang MH, Wang MX, Jin LM, Xiao ZX, Qian JC, Wang Y, Zuo W, Huang LJ, Liang G.
MD2 deficiency prevents high-fat diet-induced AMPK suppression and lipid accumulation through regulating TBK1 in non-alcoholic fatty liver disease.
Clin Transl Med 12(3) e777(2022) 35343085

Rajamanickam V, Yan T, Xu S, Hui J, Xu X, Ren L, Liu Z, Liang G, Wang O, Wang Y.
Selective targeting of the TLR4 co-receptor, MD2, prevents colon cancer growth and lung metastasis.
Int J Biol Sci 16(8) 1288-1302(2020) 32210720

Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G.
Inhibition of MD2-dependent inflammation attenuates the progression of non-alcoholic fatty liver disease.
J Cell Mol Med 22(2) 936-947(2018) 29077272

Fang Q, Wang L, Yang D, Chen X, Shan X, Zhang Y, Lum H, Wang J, Zhong P, Liang G, Wang Y.
Blockade of myeloid differentiation protein 2 prevents obesity-induced inflammation and nephropathy.
J Cell Mol Med 21(12) 3776-3786(2017) 28767204

Wang Y, Luo W, Han J, Khan ZA, Fang Q, Jin Y, Chen X, Zhang Y, Wang M, Qian J, Huang W, Lum H, Wu G, Liang G.
MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy.
Nat Commun 11(1) 2148(2020) 32358497

Wang L, Han J, Shan P, You S, Chen X, Jin Y, Wang J, Huang W, Wang Y, Liang G.
MD2 Blockage Protects Obesity-Induced Vascular Remodeling via Activating AMPK/Nrf2.
Obesity (Silver Spring) 25(9) 1532-1539(2017) 28726347

Chen H, Zhang Y, Zhang W, Liu H, Sun C, Zhang B, Bai B, Wu D, Xiao Z, Lum H, Zhou J, Chen R, Liang G.
Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury.
Phytomedicine 63 152997(2019) 31254764

Chen H, Song Z, Ying S, Yang X, Wu W, Tan Q, Ju X, Wu W, Zhang X, Qu J, Wang Y.
Myeloid differentiation protein 2 induced retinal ischemia reperfusion injury via upregulation of ROS through a TLR4-NOX4 pathway.
Toxicol Lett 282 109-120(2018) 29111459

Information1

Health Report

Gene

Information2

BRC mice in Publications