Strain Data Sheet


Strain Information

BRC No.RBRC02388
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Ly96<tm1Kmiy>
Former Common nameC57BL/6-MD-2 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Strain development
Strain description
Colony maintenance
ReferencesNat. Immunol. 3, 667-672 (2002) 12055629

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ly96lymphocyte antigen 961Ly96<tm1Kmiy>targeted mutation 1, Kensuke MiyakeESOP-1, MD-2, MD2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Ly96 genome DNA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Immunology, 3, 667-672 (2002).
DepositorKensuke Miyake (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Fang Q, Wang J, Zhang Y, Wang L, Li W, Han J, Huang W, Liang G, Wang Y.
Inhibition of myeloid differentiation factor-2 attenuates obesity-induced cardiomyopathy and fibrosis.
Biochim Biophys Acta Mol Basis Dis 1864(1) 252-262(2018) 28965884

Wang M, Zhang Q, Lou S, Jin L, Wu G, Wu W, Tang Q, Wang Y, Long X, Huang P, Luo W, Liang G.
Inhibition of MD2 by natural product-drived JM-9 attenuates renal inflammation and diabetic nephropathy in mice.
Biomed Pharmacother 168 115660(2023) 37806092

Wang Y, Fang Q, Jin Y, Liu Z, Zou C, Yu W, Li W, Shan X, Chen R, Khan Z, Liang G.
Blockade of myeloid differentiation 2 attenuates diabetic nephropathy by reducing activation of the renin-angiotensin system in mouse kidneys.
Br J Pharmacol 176(14) 2642-2657(2019) 30959575

Wang Y, Shan X, Chen G, Jiang L, Wang Z, Fang Q, Liu X, Wang J, Zhang Y, Wu W, Liang G.
MD-2 as the target of a novel small molecule, L6H21, in the attenuation of LPS-induced inflammatory response and sepsis.
Br J Pharmacol 172(17) 4391-405(2015) 26076332

Luo W, Ye L, Hu XT, Wang MH, Wang MX, Jin LM, Xiao ZX, Qian JC, Wang Y, Zuo W, Huang LJ, Liang G.
MD2 deficiency prevents high-fat diet-induced AMPK suppression and lipid accumulation through regulating TBK1 in non-alcoholic fatty liver disease.
Clin Transl Med 12(3) e777(2022) 35343085

Rajamanickam V, Yan T, Xu S, Hui J, Xu X, Ren L, Liu Z, Liang G, Wang O, Wang Y.
Selective targeting of the TLR4 co-receptor, MD2, prevents colon cancer growth and lung metastasis.
Int J Biol Sci 16(8) 1288-1302(2020) 32210720

Zhang Y, Wu B, Zhang H, Ge X, Ying S, Hu M, Li W, Huang Y, Wang L, Chen C, Shan X, Liang G.
Inhibition of MD2-dependent inflammation attenuates the progression of non-alcoholic fatty liver disease.
J Cell Mol Med 22(2) 936-947(2018) 29077272

Fang Q, Wang L, Yang D, Chen X, Shan X, Zhang Y, Lum H, Wang J, Zhong P, Liang G, Wang Y.
Blockade of myeloid differentiation protein 2 prevents obesity-induced inflammation and nephropathy.
J Cell Mol Med 21(12) 3776-3786(2017) 28767204

Wang Y, Luo W, Han J, Khan ZA, Fang Q, Jin Y, Chen X, Zhang Y, Wang M, Qian J, Huang W, Lum H, Wu G, Liang G.
MD2 activation by direct AGE interaction drives inflammatory diabetic cardiomyopathy.
Nat Commun 11(1) 2148(2020) 32358497

Wang L, Han J, Shan P, You S, Chen X, Jin Y, Wang J, Huang W, Wang Y, Liang G.
MD2 Blockage Protects Obesity-Induced Vascular Remodeling via Activating AMPK/Nrf2.
Obesity (Silver Spring) 25(9) 1532-1539(2017) 28726347

Chen H, Zhang Y, Zhang W, Liu H, Sun C, Zhang B, Bai B, Wu D, Xiao Z, Lum H, Zhou J, Chen R, Liang G.
Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury.
Phytomedicine 63 152997(2019) 31254764

Chen H, Song Z, Ying S, Yang X, Wu W, Tan Q, Ju X, Wu W, Zhang X, Qu J, Wang Y.
Myeloid differentiation protein 2 induced retinal ischemia reperfusion injury via upregulation of ROS through a TLR4-NOX4 pathway.
Toxicol Lett 282 109-120(2018) 29111459