RBRC02385

Information1

Image
BRC No.RBRC02385
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Rps6kb1<tm1Nte>
Former Common namep70s6 Knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development
Strain description
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 95, 5033-5038 (1998). 9560223

Health Report

Examination Date / Room / Rack2012/09/24Room:4-BRack:G
2012/07/23Room:4-BRack:G
2012/05/21Room:4-BRack:G
2012/03/26Room:4-BRack:G
2012/01/23Room:4-BRack:G
2011/11/28Room:4-BRack:G
2011/09/26Room:4-BRack:G
2011/07/25Room:4-BRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Rps6kb1ribosomal protein S6 kinase, polypeptide 111Rps6kb1<tm1Nte>targeted mutation 1, Naohiro Terada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Rps6kb1 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Use of the BIOLOGICAL RESOURCE or descendants by for-profit entities for Commercial Purposes requires a use license from DEPOSITOR. RIKEN BRC agrees to inquire whether the party requesting the BIOLOGICAL RESOURCE is a for-profit entity or is using the BIOLOGICAL RESOURCE of descendants for Commercial Purposes, and if so, will advise the party that it must first obtain a use license from DEPOSITOR before receiving the BIOLOGICAL RESOURCE.
Commercial Purposes shall include, but not be limited to, uses of the BIOLOGICAL RESOURCE of descendants by any organization, including recipient, to perform contract research, to screen compound libraries, to produce or manufacture products for general sale, or to conduct research activities that result in any sale, lease, license, or transfer of industrially sponsored academic research shall not be considered a use of the BIOLOGICAL RESOURCE for Commercial Purposes per se, unless any of the above conditions of this definition are met.
DepositorErwin W. Gelfand (National Jewish Medical and Research Center)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Zhao J, Li SW, Gong QQ, Ding LC, Jin YC, Zhang J, Gao JG, Sun XY.
A disputed evidence on obesity: comparison of the effects of Rcan2(-/-) and Rps6kb1(-/-) mutations on growth and body weight in C57BL/6J mice.
J Zhejiang Univ Sci B 17 657(2016) 27604858