Strain Data Sheet


Strain Information

BRC No.RBRC02385
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Rps6kb1<tm1Nte>
Former Common namep70s6 Knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6NCrlCrlj
Strain developmentDeveloped by Hideki Kawasome, Natonal Jewish Medical and Research Center in 1997.
Strain descriptionHomozygous mutant mice are small.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 95, 5033-5038 (1998). 9560223

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rps6kb1ribosomal protein S6 kinase, polypeptide 111Rps6kb1<tm1Nte>targeted mutation 1, Naohiro Terada2610318I15Rik, p70/85s6k, p70s6k, S6K1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Rps6kb1 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Use of the BIOLOGICAL RESOURCE or descendants by for-profit entities for Commercial Purposes requires a use license from DEPOSITOR. RIKEN BRC agrees to inquire whether the party requesting the BIOLOGICAL RESOURCE is a for-profit entity or is using the BIOLOGICAL RESOURCE of descendants for Commercial Purposes, and if so, will advise the party that it must first obtain a use license from DEPOSITOR before receiving the BIOLOGICAL RESOURCE.
Commercial Purposes shall include, but not be limited to, uses of the BIOLOGICAL RESOURCE of descendants by any organization, including recipient, to perform contract research, to screen compound libraries, to produce or manufacture products for general sale, or to conduct research activities that result in any sale, lease, license, or transfer of industrially sponsored academic research shall not be considered a use of the BIOLOGICAL RESOURCE for Commercial Purposes per se, unless any of the above conditions of this definition are met.
DepositorErwin W. Gelfand (National Jewish Medical and Research Center)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Zhao J, Li SW, Gong QQ, Ding LC, Jin YC, Zhang J, Gao JG, Sun XY.
A disputed evidence on obesity: comparison of the effects of Rcan2(-/-) and Rps6kb1(-/-) mutations on growth and body weight in C57BL/6J mice.
J Zhejiang Univ Sci B 17(9) 657-71(2016) 27604858