Strain Data Sheet

RBRC02382

Information1

Image
BRC No.RBRC02382
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CXCL14)20
Former Common namehBRAKlongExp20L, C57BL/6J-Tg(CXCL14)1
H-2 Haplotype
ES Cell line
Background strainC57BL/6J
Appearance
Strain developmentDeveloped by Ryu-Ichiro Hata, Kanagawa Dental College in 2007.
Strain descriptionThis transgenic mouse expresses the human chemokine (C-X-C motif) ligand 14 gene under he control of CAG promoter. line 20. Level of CXCL14/BRAK is 14 ng/ml plasma (in homozygote).
Colony maintenance
ReferencesTransgenic Res., 19, 1109-1117 (2010). 20333465
Biochem. Biophys. Res. Commun., 348, 406-412 (2006). 16884687

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CXCL14chemokine (C-X-C motif) ligand 14UNCXCL14CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ApolyAUNpoly A

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Human BRAKLong cDNA, SV40Late polyA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorRyu-Ichiro Hata (Kanagawa Dental College)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Ishida Y, Kimura A, Nosaka M, Kuninaka Y, Hemmi H, Sasaki I, Kaisho T, Mukaida N, Kondo T.
Essential involvement of the CX3CL1-CX3CR1 axis in bleomycin-induced pulmonary fibrosis via regulation of fibrocyte and M2 macrophage migration.
Sci Rep 7(1) 16833(2017) 29203799