Strain Data Sheet

RBRC02370

Strain Information

Image
BRC No.RBRC02370
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Grn<tm1Mni>
Former Common nameProgranulin knock-out mouse, B6;129-Grn<tm1>
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masugi Nishihara, Veterinary Medical Science, The University of Tokyo and Yoichiro Iwakura, Institute of Medical Science, The University of Tokyo in 2001. Targeting construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd.
Strain descriptionGrn, granulin knockout mice. A 4.7 kb fragment encoding exon 2-13 of Grn gene was replaced with a PGK-neo-bpA cassette. Homozygous mutant mice show a decrease in ejaculation incidence, enhanced aggressiveness and an elevated level of anxiety in male.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc].
References
Alteration of behavioural phenotype in mice by targeted disruption of the progranulin gene.
Kayasuga Y, Chiba S, Suzuki M, Kikusui T, Matsuwaki T, Yamanouchi K, Kotaki H, Horai R, Iwakura Y, Nishihara M
Behav. Brain Res., 185, 110-118 (2007). 17764761

Health Report

Examination Date / Room / Rack2024/06/10Room:3-3Rack:DSentinel mouse program
2024/03/11Room:3-3Rack:DSentinel mouse program
2023/12/11Room:3-3Rack:DSentinel mouse program
2023/09/11Room:3-3Rack:DSentinel mouse program
2023/06/13Room:3-3Rack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)11GH
Grn
MGI:95832		granulin11Grn<tm1Mni>
MGI:3762618		targeted mutation1, Masugi Nishihara
  • frontotemporal dementia(DisGeNET)

  • GRN-related frontotemporal lobar degeneration with Tdp43 inclusions(MedGEN)

  • neuronal ceroid lipofuscinosis 11(MedGEN)
    • neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal ejaculation(MP:0001378)

  • increased aggression towards male mice(MP:0001354)

  • increased anxiety-related response(MP:0001363)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Bovine growth hormone poly A signal, mouse Grn genomic DNA
    Research applicationNeurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Behav. Brain. Res., 185, 110-118 (2007).
    DepositorMasugi　Nishihara (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Adachi E, Murakoshi M, Shibata T, Shimozawa K, Sakuma H, Kishida C, Gohda T, Suzuki Y.
    Progranulin deficiency attenuates tubulointerstitial injury in a mouse unilateral ureteral obstruction model.
    Exp Anim (2024) 38369347
    Tsuji S, Kudo U, Takahashi K, Nakamura S, Shimazawa M.
    The role of progranulin in macrophages of a glioblastoma model.
    J Neurooncol (2024) 39141257
    Takahashi H, Bhagwagar S, Nies SH, Ye H, Han X, Chiasseu MT, Wang G, Mackenzie IR, Strittmatter SM.
    Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase.
    Nat Commun 15(1) 1434(2024) 38365772
    Sasaki T, Kuse Y, Nakamura S, Shimazawa M.
    Progranulin-deficient macrophages cause cardiotoxicity under hypoxic conditions.
    Biochem Biophys Res Commun 691 149341(2023) 38039836
    Sasaki T, Kuse Y, Nakamura S, Shimazawa M, Hara H.
    Progranulin deficiency exacerbates cardiac remodeling after myocardial infarction.
    FASEB Bioadv 5(10) 395-411(2023) 37810172
    Nies SH, Takahashi H, Herber CS, Huttner A, Chase A, Strittmatter SM.
    Spreading of Alzheimer tau seeds is enhanced by aging and template matching with limited impact of amyloid-β.
    J Biol Chem 297(4) 101159(2021) 34480901
    Murakoshi M, Gohda T, Adachi E, Ichikawa S, Hagiwara S, Suzuki Y.
    Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice.
    Sci Rep 11(1) 1194(2021) 33441916
    Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M.
    Microglia increases the proliferation of retinal precursor cells during postnatal development.
    Mol Vis 24 536-545(2018) 30090016
    Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H.
    Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina.
    Sci Rep 6 23811(2016) 27030285
    Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, Yoshikawa H.
    Progranulin plays crucial roles in preserving bone mass by inhibiting TNF-α-induced osteoclastogenesis and promoting osteoblastic differentiation in mice.
    Biochem Biophys Res Commun 465(3) 638-43(2015) 26297947
    Hosokawa M, Arai T, Masuda-Suzukake M, Kondo H, Matsuwaki T, Nishihara M, Hasegawa M, Akiyama H.
    Progranulin reduction is associated with increased tau phosphorylation in P301L tau transgenic mice.
    J Neuropathol Exp Neurol 74(2) 158-65(2015) 25575133
    Hafler BP, Klein ZA, Jimmy Zhou Z, Strittmatter SM.
    Progressive retinal degeneration and accumulation of autofluorescent lipopigments in Progranulin deficient mice.
    Brain Res 1588 168-74(2014) 25234724
    Kawase R, Ohama T, Matsuyama A, Matsuwaki T, Okada T, Yamashita T, Yuasa-Kawase M, Nakaoka H, Nakatani K, Inagaki M, Tsubakio-Yamamoto K, Masuda D, Nakagawa-Toyama Y, Nishida M, Ohmoto Y, Nishihara M, Komuro I, Yamashita S.
    Deletion of progranulin exacerbates atherosclerosis in ApoE knockout mice.
    Cardiovasc Res 100(1) 125-33(2013) 23847387