Strain Data Sheet
RBRC02370
Information1 | |
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| Image |  |
| BRC No. | RBRC02370 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129-Grn<tm1Mni> |
| Former Common name | Progranulin knock-out mouse, B6;129-Grn<tm1> |
| H-2 Haplotype | |
| ES Cell line | E14.1 [129P2/OlaHsd] |
| Background strain | C57BL/6 |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Masugi Nishihara, Veterinary Medical Science, The University of Tokyo and Yoichiro Iwakura, Institute of Medical Science, The University of Tokyo in 2001. Targeting construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. |
| Strain description | Grn, granulin knockout mice. A 4.7 kb fragment encoding exon 2-13 of Grn gene was replaced with a PGK-neo-bpA cassette. Homozygous mutant mice show a decrease in ejaculation incidence, enhanced aggressiveness and an elevated level of anxiety in male. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJmsSlc]. |
| References | Behav. Brain Res., 185, 110-118 (2007). 17764761 |
Health Report | |
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| Examination Date / Room / Rack | 2023/06/13Room:3-3Rack:D |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Grngranulin11Grn<tm1Mni>targeted mutation1, Masugi Nishiharaacrogranulin, epithelin, PC cell-derived growth factor, progranulin Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)11GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Information2 | |
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| Donor DNA | mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Bovine growth hormone poly A signal, mouse Grn genomic DNA |
| Research application | Neurobiology Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Behav. Brain. Res., 185, 110-118 (2007). |
| Depositor | Masugi Nishihara (The University of Tokyo) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Genetic Background Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, Yoshikawa H. Progranulin plays crucial roles in preserving bone mass by inhibiting TNF-α-induced osteoclastogenesis and promoting osteoblastic differentiation in mice. Biochem Biophys Res Commun 465(3) 638-43(2015) 26297947 Hafler BP, Klein ZA, Jimmy Zhou Z, Strittmatter SM. Progressive retinal degeneration and accumulation of autofluorescent lipopigments in Progranulin deficient mice. Brain Res 1588 168-74(2014) 25234724 Kawase R, Ohama T, Matsuyama A, Matsuwaki T, Okada T, Yamashita T, Yuasa-Kawase M, Nakaoka H, Nakatani K, Inagaki M, Tsubakio-Yamamoto K, Masuda D, Nakagawa-Toyama Y, Nishida M, Ohmoto Y, Nishihara M, Komuro I, Yamashita S. Deletion of progranulin exacerbates atherosclerosis in ApoE knockout mice. Cardiovasc Res 100(1) 125-33(2013) 23847387 Nies SH, Takahashi H, Herber CS, Huttner A, Chase A, Strittmatter SM. Spreading of Alzheimer Tau seeds is enhanced by aging and template matching with limited impact of amyloid-β. J Biol Chem 101159(2021) 34480901 Hosokawa M, Arai T, Masuda-Suzukake M, Kondo H, Matsuwaki T, Nishihara M, Hasegawa M, Akiyama H. Progranulin reduction is associated with increased tau phosphorylation in P301L tau transgenic mice. J Neuropathol Exp Neurol 74(2) 158-65(2015) 25575133 Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M. Microglia increases the proliferation of retinal precursor cells during postnatal development. Mol Vis 24 536-545(2018) 30090016 Murakoshi M, Gohda T, Adachi E, Ichikawa S, Hagiwara S, Suzuki Y. Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice. Sci Rep 11(1) 1194(2021) 33441916 Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H. Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina. Sci Rep 6 23811(2016) 27030285 |