Strain Data Sheet

RBRC02370

Strain Information

Image
BRC No.RBRC02370
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Grn<tm1Mni>
Former Common nameProgranulin knock-out mouse, B6;129-Grn<tm1>
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masugi Nishihara, Veterinary Medical Science, The University of Tokyo and Yoichiro Iwakura, Institute of Medical Science, The University of Tokyo in 2001. Targeting construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd.
Strain descriptionGrn, granulin knockout mice. A 4.7 kb fragment encoding exon 2-13 of Grn gene was replaced with a PGK-neo-bpA cassette. Homozygous mutant mice show a decrease in ejaculation incidence, enhanced aggressiveness and an elevated level of anxiety in male.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc].
ReferencesBehav. Brain Res., 185, 110-118 (2007). 17764761

Health Report

Examination Date / Room / Rack2024/06/10Room:3-3Rack:D
2024/03/11Room:3-3Rack:D
2023/12/11Room:3-3Rack:D
2023/09/11Room:3-3Rack:D
2023/06/13Room:3-3Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Grngranulin11Grn<tm1Mni>targeted mutation1, Masugi Nishiharaacrogranulin, epithelin, PC cell-derived growth factor, progranulin

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Bovine growth hormone poly A signal, mouse Grn genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Behav. Brain. Res., 185, 110-118 (2007).
DepositorMasugi Nishihara (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Sasaki T, Kuse Y, Nakamura S, Shimazawa M.
Progranulin-deficient macrophages cause cardiotoxicity under hypoxic conditions.
Biochem Biophys Res Commun 691 149341(2023) 38039836

Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, Yoshikawa H.
Progranulin plays crucial roles in preserving bone mass by inhibiting TNF-α-induced osteoclastogenesis and promoting osteoblastic differentiation in mice.
Biochem Biophys Res Commun 465(3) 638-43(2015) 26297947

Hafler BP, Klein ZA, Jimmy Zhou Z, Strittmatter SM.
Progressive retinal degeneration and accumulation of autofluorescent lipopigments in Progranulin deficient mice.
Brain Res 1588 168-74(2014) 25234724

Kawase R, Ohama T, Matsuyama A, Matsuwaki T, Okada T, Yamashita T, Yuasa-Kawase M, Nakaoka H, Nakatani K, Inagaki M, Tsubakio-Yamamoto K, Masuda D, Nakagawa-Toyama Y, Nishida M, Ohmoto Y, Nishihara M, Komuro I, Yamashita S.
Deletion of progranulin exacerbates atherosclerosis in ApoE knockout mice.
Cardiovasc Res 100(1) 125-33(2013) 23847387

Adachi E, Murakoshi M, Shibata T, Shimozawa K, Sakuma H, Kishida C, Gohda T, Suzuki Y.
Progranulin deficiency attenuates tubulointerstitial injury in a mouse unilateral ureteral obstruction model.
Exp Anim (2024) 38369347

Sasaki T, Kuse Y, Nakamura S, Shimazawa M, Hara H.
Progranulin deficiency exacerbates cardiac remodeling after myocardial infarction.
FASEB Bioadv 5(10) 395-411(2023) 37810172

Nies SH, Takahashi H, Herber CS, Huttner A, Chase A, Strittmatter SM.
Spreading of Alzheimer tau seeds is enhanced by aging and template matching with limited impact of amyloid-β.
J Biol Chem 297(4) 101159(2021) 34480901

Hosokawa M, Arai T, Masuda-Suzukake M, Kondo H, Matsuwaki T, Nishihara M, Hasegawa M, Akiyama H.
Progranulin reduction is associated with increased tau phosphorylation in P301L tau transgenic mice.
J Neuropathol Exp Neurol 74(2) 158-65(2015) 25575133

Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M.
Microglia increases the proliferation of retinal precursor cells during postnatal development.
Mol Vis 24 536-545(2018) 30090016

Takahashi H, Bhagwagar S, Nies SH, Ye H, Han X, Chiasseu MT, Wang G, Mackenzie IR, Strittmatter SM.
Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase.
Nat Commun 15(1) 1434(2024) 38365772

Murakoshi M, Gohda T, Adachi E, Ichikawa S, Hagiwara S, Suzuki Y.
Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice.
Sci Rep 11(1) 1194(2021) 33441916

Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H.
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina.
Sci Rep 6 23811(2016) 27030285