BRC No.RBRC02370
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Grn<tm1>
Former Common nameProgranulin knock-out mouse
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masugi Nishihara, Veterinary Medical Science, The University of Tokyo and Yoichiro Iwakura, Institute of Medical Science, The University of Tokyo in 2001. Targeting construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd.
Strain descriptionGrn, granulin knockout mice. A 4.7 kb fragment encoding exon 2-13 of Grn gene was replaced with a PGK-neo-bpA cassette. Homozygous mutant mice show a decrease in ejaculation incidence, enhanced aggressiveness and an elevated level of anxiety in male.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc].
ReferencesBehav. Brain Res., 185, 110-118 (2007). 17764761

Health Report

Examination Date / Room / Rack2013/11/25Room:4-ARack:E


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Grngranulin11Grn<tm1Mni>targeted mutation1, Masugi Nishihara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)


Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, Bovine growth hormone poly A signal, mouse Grn genomic DNA
Research applicationNeurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Behav. Brain. Res., 185, 110-118 (2007).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorMasugi Nishihara (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, Yoshikawa H.
Progranulin plays crucial roles in preserving bone mass by inhibiting TNF- -induced osteoclastogenesis and promoting osteoblastic differentiation in mice.
Biochem. Biophys. Res. Commun. (2015) 26297947

Hafler BP, Klein ZA, Jimmy Zhou Z, Strittmatter SM.
Progressive retinal degeneration and accumulation of autofluorescent lipopigments in Progranulin deficient mice.
Brain Res. (2014) 25234724

Kawase R, Ohama T, Matsuyama A, Matsuwaki T, Okada T, Yamashita T, Yuasa-Kawase M, Nakaoka H, Nakatani K, Inagaki M, Tsubakio-Yamamoto K, Masuda D, Nakagawa-Toyama Y, Nishida M, Ohmoto Y, Nishihara M, Komuro I, Yamashita S.
Deletion of progranulin exacerbates atherosclerosis in ApoE knockout mice.
Cardiovasc. Res. 100(1) 125-33(2013) 23847387

Hosokawa M, Arai T, Masuda-Suzukake M, Kondo H, Matsuwaki T, Nishihara M, Hasegawa M, Akiyama H.
Progranulin reduction is associated with increased tau phosphorylation in P301L tau transgenic mice.
J. Neuropathol. Exp. Neurol. 74(2) 158-65(2015) 25575133

Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M.
Microglia increases the proliferation of retinal precursor cells during postnatal development.
Mol. Vis. 24 536-545.(2018) 30090016

Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H.
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina.
Sci Rep (2016) 27030285