Strain Data Sheet

RBRC02369

Information1

Image
BRC No.RBRC02369
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Rsph1<tm1Htan>
Former Common name
H-2 Haplotype
ES Cell lineW9.5 [129S1/Sv-Oca2<+> Tyr<+> Kitl<+>]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Biol. Chem., 283(27):19039-19048(2008). 18453535

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rsph1radical spoke head 1 homolog (Chlamydomonas)17Rsph1<tm1Htan>targeted mutation 1, Hiromitsu Tanakameichroacidin, Tsga2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli neo, mouse Rsph1(meichroacidin) genomic DNA
Research applicationDevelopmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHiromitsu Tanaka (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

Yin W, Livraghi-Butrico A, Sears PR, Rogers TD, Burns KA, Grubb BR, Ostrowski LE.
Mice with a Deletion of Rsph1 Exhibit a Low Level of Mucociliary Clearance and Develop a Primary Ciliary Dyskinesia Phenotype.
Am J Respir Cell Mol Biol 61(3) 312-321(2019) 30896965