RBRC02348

Information1

Image
BRC No.RBRC02348
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Fabp7<tm1(crePR)Ksak>
Former Common nameBFABP-CrePR
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Mika Tsujita, Rie Natsume and Kenji Sakimura, Brain Research Institute, Niigata University in 2004. RENKA ES cells derived from C57BL/6NCrj were used. C57BL/6N background.
Strain descriptionFabp7 CrePR knock-in mice. A CrePR sequence was integrated into the start methionine site of the B-FABP gene at an in-frame position. A non-coding and pgk-neo cassette sequence are located downstream from CrePR. The pgk-neo cassette can be removed, as it has an frt sequence on both sides.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack2008/07/14Room:4-ARack:L
2008/05/20Room:4-ARack:L
2008/05/19Room:4-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Fabp7fatty acid binding protein 7, brain10Fabp7<tm1(crePR)Ksak>

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
PGRProgesterone receptor (human)10PGR

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase10cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly Amouse pgk polyA10poly A

Information2

Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre, human progesterone receptor cDNA, human G-CSF polyadenylation signal sequence (pA), mouse phosphoglycerokinase (Pgk1) promoter, E. coli neo, mouse Pgk1 poly A, S. cerevisiae 2 micro plasmid FRT sites
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
General Purpose
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-auther in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice.
DepositorKenji Sakimura (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data