RBRC02340

Information1

Image
BRC No.RBRC02340
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(bitetO-GFP)BYoko
Former Common namebitetO-GFP
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Columbia University in 2002. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionThis transgenic mice express a bitetO-GFP contained the bidirectional tetracycline operator (tetO) promoter driving EGFP and a rabbit beta-globin intron/polyA signal in one direction, and in the opposite direction, driving an artifical intron, an artificial fusion gene consisting of a placental alkaline phosphatase fragment, tetanus toxin C fragment, tyrosine phosphatase delta fragment, and glutamate receptor 2 fragment (RT8) and an SV40 polyadenylation signal. This strain can be used to make an inducible expression of EGFP crossing with the genetically-engineered mice that have a tetracycline-controlled transactivator (tTA).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]Tg/+ hemizygous mice are viable and fertile.
ReferencesJ. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack2010/11/16Room:4-ARack:E
2010/09/13Room:4-ARack:E
2010/07/12Room:4-ARack:E
2010/05/17Room:4-ARack:E
2010/03/15Room:4-ARack:E
2010/01/18Room:4-ARack:E
2009/11/16Room:4-ARack:E
2009/09/14Room:4-ARack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gria2glutamate receptor, ionotropic, AMPA2 (alpha 2)UNGria2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ptprdprotein tyrosine phosphatase, receptor type, DUNPtprd

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UNE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin intron/polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
PLAPhuman placental alkaline phosphataseUNPLAP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TTCNon-toxic c fragment (Clostridium tetani)UNTTC

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ASV40 polyA signalUNpoly A

Information2

Donor DNAE. coli tetO, CMV minimal promoter, Aequoria Victoria GFP cDNA, Rabbit beta-Globin intron/polyA signal, Human placental alkaline phosphatase, Clostridium tetani Non-toxic c fragment, Mouse tyrosine phosphatase δ(fragment), Mouse glutamate receptor 2 (fragment), SV40 polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 6948-6955 (2007).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Mayumi Yamada, Yusuke Suzuki, Shinji C.Nagasaki, Hiroyuki Okuno, Itaru Imayoshi.
Light Control of the Tet Gene Expression System in Mammalian Cells.
Cell Rep 25(2) 487-500.(2018) 30304687