Strain Data Sheet


Strain Information

BRC No.RBRC02338
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-p130Cas<tm2.1Homy>
Former Common nameCas-FL conditional KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Hiroaki Honda, Hiroshima University in 2005.
Strain descriptionBcar1 (p130Cas) is an adaptor molecule on growth factor receptor and integrin activation functions as regulator of cell motility and invasion. This conditional knockout mice have a loxP-flanked Bcar1 allele. Conditional Bcar1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenancebackcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj)
ReferencesJ. Bone Miner. Res., 28(12):2449-62 (2013). 23526406
Am. J. Physiol. Cell Physiol., 304(6):C541-7 (2013). 23325412

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bcar1breast cancer anti-estrogen resistance 18Bcar1<tm2.1Homy>targeted mutation 2.1, Hiroaki HondaCas, Crkas, p130 Cas

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP8loxP

Ordering Information

Donor DNAphage P1 loxP sites, rat Cas cDNA, SV40 poly A, yeast FRT site, mouse Cas genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Akimoto T, Okuhira K, Aizawa K, Wada S, Honda H, Fukubayashi T, Ushida T.
Skeletal muscle adaptation in response to mechanical stress in p130cas-/- mice.
Am J Physiol Cell Physiol 304(6) C541-7(2013) 23325412

Miyazaki T, Zhao Z, Ichihara Y, Yoshino D, Imamura T, Sawada K, Hayano S, Kamioka H, Mori S, Hirata H, Araki K, Kawauchi K, Shigemoto K, Tanaka S, Bonewald LF, Honda H, Shinohara M, Nagao M, Ogata T, Harada I, Sawada Y.
Mechanical regulation of bone homeostasis through p130Cas-mediated alleviation of NF-κB activity.
Sci Adv 5(9) eaau7802(2019) 31579816