Strain Data Sheet

RBRC02337

Strain Information

Image
BRC No.RBRC02337
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Top2a<tm1Skmz>
Former Common nameTopoisomerase II aloha knockout mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6
Appearance
Strain development
Strain description
Colony maintenance
References
Enforced cytokinesis without complete nuclear division in embryonic cells depleting the activity of DNA topoisomerase IIalpha.
Akimitsu N, Adachi N, Hirai H, Hossain M S, Hamamoto H, Kobayashi M, Aratani Y, Koyama H, Sekimizu K
Genes Cells, 8, 393-402 (2003) 12653966

Decreased topoisomerase IIalpha expression confers increased resistance to ICRF-193 as well as VP-16 in mouse embryonic stem cells.
Kobayashi M, Adachi N, Aratani Y, Kikuchi A, Koyama H
Cancer Lett., 166, 714-77 (2001). 11295289

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Top2atopoisomerase (DNA) II alpha11Top2atargeted mutation 1, Kazuhisa Sekimizu
neoneomycin resistance gene (E. coli)11

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • embryonic lethality(MP:0008762)
    • Detailed phenotype data

    Ordering Information

    Donor DNAneomycin耐性遺伝子, mouse TopoisomeraseII alpha
    Research applicationCancer Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells, 8, 393-402 (2003). Cancer Lett., 166, 71-77 (2001)The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorKazuhisa Sekimizu (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data