RBRC02332

Information1

Image
BRC No.RBRC02332
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Drd2-YFP)364-5
Former Common nameB6.Cg-Tg(Drd2-YFP)364-5
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 2007. The Drd2-YFP transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionThis transgenic strain expresses YFP under the control of rat dopamine receptor 2 gene promoter.
Colony maintenanceHemizygote x Non carrier [C57BL/6JJcl]
ReferencesNeuron, 89 (3) 550-565 (2016). 26804993

Health Report

Examination Date / Room / Rack2012/11/05Room:2-3Rack:C
2012/09/03Room:2-3Rack:C
2012/07/02Room:2-3Rack:C
2012/05/07Room:2-3Rack:C
2008/07/07Room:4-5Rack:C
2008/05/13Room:4-5Rack:C
2008/05/12Room:4-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
YFPYellow Fluorescent Protein (Jellyfish)UNYFPrat Drd2 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ASV40 polyAUNpoly A

Information2

Donor DNARat Drd2 promoter (Rat BAC clone), Jellyfish YFP cDNA、SV40 early gene polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 89 (3) 550-565 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall contact the DEPOSITOR (Dr. Kazuto Kobayasi, kazuto@fmu.ac.jp) about the use of the BIOLOGICAL RESOURCE in advance.
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

Nagai T, Nakamuta S, Kuroda K, Nakauchi S, Nishioka T, Takano T, Zhang X, Tsuboi D, Funahashi Y, Nakano T, Yoshimoto J, Kobayashi K, Uchigashima M, Watanabe M, Miura M, Nishi A, Kobayashi K, Yamada K, Amano M, Kaibuchi K.
Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo.
Neuron 89(3) 550-65. (2016) 26804993

Bin Saifullah MA, Nagai T, Kuroda K, Wulaer B, Nabeshima T, Kaibuchi K, Yamada K.
Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice.
Sci Rep 8(1) 14413.(2018) 30258218