Strain Data Sheet

RBRC02326

Information1

Image
BRC No.RBRC02326
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Ecat8<tm1Yam>
Former Common nameB6;129S4-Ecat8<tm1Yam>
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 110, 16492-16497 (2013). 24067652

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tdrd12tudor domain containing 127Tdrd12<tm1Yam>targeted mutation 1, Shinya Yamanaka2410004F06Rik, 2410070K17Rik, ecat8

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)7internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA7poly A

Information2

Donor DNAE. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 pA, mouse Tdrd12 genomic DNA
Research applicationCell Biology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT must obtain permission from the DEPOSITOR prior to receiving the BIOLOGICAL RESOURCE (RECIPIENT shall contact KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp for getting permission). The BIOLOGICAL RESOURCE is only for academic research purpose by the RECIPIENT who belongs to a non-profit organization. The RECIPIENT shall not transfer the BIOLOGICAL RESOURCE or the parts thereof including those contained in modification or cross-bred mouse, to any third party. The RECIPIENT shall express acknowledgment in any publication that the BIOLOGIOCAL RESOURCE was provided by Dr. Shinya Yamanaka, KYOTO UNIVERSITY.
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data