Strain Data Sheet

RBRC02326

Strain Information

Image
BRC No.RBRC02326
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S4-Ecat8<tm1Yam>
Former Common nameB6;129S4-Ecat8<tm1Yam>
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Tudor domain containing 12 (TDRD12) is essential for secondary PIWI interacting RNA biogenesis in mice.
Pandey R R, Tokuzawa Y, Yang Z, Hayashi E, Ichisaka T, Kajita S, Asano Y, Kunieda T, Sachidanandam R, Chuma S, Yamanaka S, Pillai R S
Proc. Natl. Acad. Sci. USA, 110, 16492-16497 (2013). 24067652

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
IRES internal ribosomal entry site (EMCV)7
Tdrd12
MGI:1919231		tudor domain containing 127Tdrd12<tm1Yam>
MGI:5556124		targeted mutation 1, Shinya Yamanaka
lacZ beta-galactosidase (E. coli)7
neo neomycin resistance gene (E. coli)7
poly A SV40 polyA7poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal seminiferous tubule morphology(MP:0002216)

  • abnormal sperm physiology(MP:0004543)

  • abnormal spermatid morphology(MP:0006380)

  • arrest of spermatogenesis(MP:0001155)

  • azoospermia(MP:0005159)
    • more 3 phenotypes
  • male infertility(MP:0001925)

  • small seminiferous tubules(MP:0001153)

  • testicular atrophy(MP:0003205)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, E. coli neo, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), SV40 pA, mouse Tdrd12 genomic DNA
    Research applicationCell Biology Research
    Developmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT must obtain permission from the DEPOSITOR prior to receiving the BIOLOGICAL RESOURCE (RECIPIENT shall contact KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp for getting permission). The BIOLOGICAL RESOURCE is only for academic research purpose by the RECIPIENT who belongs to a non-profit organization. The RECIPIENT shall not transfer the BIOLOGICAL RESOURCE or the parts thereof including those contained in modification or cross-bred mouse, to any third party. The RECIPIENT shall express acknowledgment in any publication that the BIOLOGIOCAL RESOURCE was provided by Dr. Shinya Yamanaka, KYOTO UNIVERSITY.
    DepositorShinya Yamanaka (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data