RBRC02323

Information1

Image
BRC No.RBRC02323
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6JNrs.129S6-Atm<tm1Awb>/NrsFnt
Former Common nameB6-Atm knock-out mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JNrs
Appearanceblack [a/a B/B C/C]
Strain developmentThe targeted alle was developed by Dr. A. Wynshow-Boris, NHI. The mutant mice were introduced into NRS in 1997, and then generated the Atm KO mice of C57BL/6 background by backcrossing to C57BL/6JNrs for 16 times.
Strain descriptionAtm, ataxia telangiectasia mutated homolog (human) gene knockout mice. Homozygous mutant mice disrupted Atm gene exhibit growth retardatuin, neurologic dysfunction, infertility, defects in T lymphocyte maturation.
Colony maintenanceSibling Mating (Heterozygote x Wild-type)
ReferencesCell, 86, 159-171 (1996). 8689683

Health Report

Examination Date / Room / Rack2018/10/15Room:3-BRack:G
2018/08/20Room:3-BRack:G
2018/06/18Room:3-BRack:G
2018/04/16Room:3-BRack:G
2018/02/19Room:3-BRack:G
2017/12/18Room:3-BRack:G
2017/10/16Room:3-BRack:G
2017/08/21Room:3-BRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Atmataxia telangiectasia mutated homolog (human)9Atm<tm1Awb>targeted mutation 1, Anthony Wynshaw Boris

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)9human phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAhuman phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse atm genomic DNA
Research applicationCancer Research
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell. 89, 159-171 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowlidgement to Dr. Kouichi Tatsumi is requested.
DepositorHideo Tsuji (National Institute of Radiological Sciences (NIRS))
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

No Data