Strain Data Sheet

RBRC02323

Strain Information

Image
BRC No.RBRC02323
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6JNrs.129S6-Atm<tm1Awb>/NrsFnt
Former Common nameB6-Atm knock-out mice
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strainC57BL/6JNrs
Appearanceblack [a/a B/B C/C]
Strain developmentThe targeted alle was developed by Dr. A. Wynshow-Boris, NHI. The mutant mice were introduced into NRS in 1997, and then generated the Atm KO mice of C57BL/6 background by backcrossing to C57BL/6JNrs for 16 times.
Strain descriptionAtm, ataxia telangiectasia mutated homolog (human) gene knockout mice. Homozygous mutant mice disrupted Atm gene exhibit growth retardatuin, neurologic dysfunction, infertility, defects in T lymphocyte maturation.
Colony maintenanceSibling Mating (Heterozygote x Wild-type)
References
Atm-deficient mice: a paradigm of ataxia telangiectasia.
Barlow C, Hirotsune S, Paylor R, Liyanage M, Eckhaus M, Collins F, Shiloh Y, Crawley J N, Ried T, Tagle D, Wynshaw-Boris A
Cell, 86, 159-171 (1996). 8689683

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Atmataxia telangiectasia mutated9Atmtargeted mutation 1, Anthony Wynshaw Boris
  • ataxia telangiectasia(DisGeNET, MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • leukemia(DisGeNET)
    • more 1 Diseases
  • lymphoma(DisGeNET)
    • neoneomycin resistance gene (E. coli)9human phosphoglycerate kinase promoter (PGK promoter)
  • ataxia telangiectasia(DisGeNET, MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • leukemia(DisGeNET)
    • more 1 Diseases
  • lymphoma(DisGeNET)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Purkinje cell morphology(MP:0000877)

  • abnormal cell cycle checkpoint function(MP:0004045)

  • abnormal cell cycle(MP:0003077)

  • abnormal chromosome morphology(MP:0003702)

  • abnormal female reproductive system morphology(MP:0001119)
    • more 43 phenotypes
  • abnormal gametogenesis(MP:0001929)

  • abnormal lysosome morphology(MP:0005058)

  • abnormal male reproductive system morphology(MP:0001145)

  • abnormal reproductive system physiology(MP:0001919)

  • abnormal seminiferous tubule morphology(MP:0002216)

  • abnormal tumor incidence(MP:0002019)

  • abnormal tumor susceptibility(MP:0002166)

  • abnormal uterus morphology(MP:0001120)

  • absent estrous cycle(MP:0009009)

  • absent estrus(MP:0009021)

  • absent oocytes(MP:0004805)

  • absent ovarian follicles(MP:0002777)

  • arrest of spermatogenesis(MP:0001155)

  • azoospermia(MP:0005159)

  • cellular phenotype(MP:0005384)

  • chromosome breakage(MP:0004028)

  • decreased T cell number(MP:0005018)

  • decreased activated T cell number(MP:0001830)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased double-positive T cell number(MP:0005092)

  • decreased fibroblast proliferation(MP:0011704)

  • decreased locomotor activity(MP:0001402)

  • decreased vertical activity(MP:0002757)

  • female infertility(MP:0001926)

  • immune system phenotype(MP:0005387)

  • impaired coordination(MP:0001405)

  • increased B cell number(MP:0005014)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased cellular sensitivity to gamma-irradiation(MP:0002007)

  • increased double-positive T cell number(MP:0005091)

  • increased hepatocellular carcinoma incidence(MP:0003331)

  • increased mortality induced by gamma-irradiation(MP:0001658)

  • lethality, incomplete penetrance(MP:0010831)

  • male infertility(MP:0001925)

  • mortality/aging(MP:0010768)

  • nervous system phenotype(MP:0003631)

  • oxidative stress(MP:0003674)

  • premature death(MP:0002083)

  • short stride length(MP:0001407)

  • small ovary(MP:0001127)

  • small testis(MP:0001147)

  • spontaneous chromosome breakage(MP:0004029)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse atm genomic DNA
    Research applicationCancer Research
    Immunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell. 89, 159-171 (1996).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowlidgement to Dr. Kouichi Tatsumi is requested.
    DepositorHideo Tsuji (National Institute of Radiological Sciences (NIRS))
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data