RBRC02322
Information1 | |
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| Image |  |
| BRC No. | RBRC02322 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Cmah<tm1Ykoz> |
| Former Common name | Cmah null mouse, KZO |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | C57BL/6JJcl |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Hiromu Takematsu and Yasunori Kozutumi, Graduate School of Biostudies, Kyoto University in 2000. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J. |
| Strain description | Cmah gene knockout mice. A neo cassette was inerted into the second coding exon. This mouse is deficient for the biosynthesis of N-glycolylneuramic acid, one of the major species of sialic acid. Due to the deficiency, sialic acid species of this mouse is almost 100% N-acetylneuraminic acid, which is the precursor of N-glycolylneuraminic acid in the biosynthetic pathway. Since CMAH is one of rare gene inactivated in human but retained in the other homonids such as chimpanzee. Thus, this mouse could by utilized to mimic human in terms of the sialic acid species expression. |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
| References | Mol. Cell. BIol., 27, 3008-3022 (2007). 17296732 |
Health Report | |
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| Examination Date / Room / Rack | 2008/05/13Room:4-5Rack:B 2008/05/12Room:4-5Rack:B |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele namePromoter Cmahcytidine monophospho-N-acetylneuraminic acid hydroxylase13Cmah<tm1Ykoz>targeted mutation 1, Yasunori Kozutsumi Gene symbolGene nameChr.Allele symbolAllele namePromoter neoneomycin resistance gene (E. coli)13 |
Information2 | |
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| Donor DNA | mouse Cmah genomic DNA, mouse PGK promoter, E.coli reomycin resistance gene |
| Research application | Metabolism Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 27, 3008-3022 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use of the BIOLOGICAL RESOURCE only for publication of research papers. |
| Depositor | Yasunori Kozutsumi (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- |
BRC mice in Publications |
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