BRC No.RBRC02322
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Cmah<tm1Ykoz>
Former Common nameCmah null mouse, KZO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Hiromu Takematsu and Yasunori Kozutumi, Graduate School of Biostudies, Kyoto University in 2000. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionCmah gene knockout mice. A neo cassette was inerted into the second coding exon. This mouse is deficient for the biosynthesis of N-glycolylneuramic acid, one of the major species of sialic acid. Due to the deficiency, sialic acid species of this mouse is almost 100% N-acetylneuraminic acid, which is the precursor of N-glycolylneuraminic acid in the biosynthetic pathway. Since CMAH is one of rare gene inactivated in human but retained in the other homonids such as chimpanzee. Thus, this mouse could by utilized to mimic human in terms of the sialic acid species expression.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesMol. Cell. BIol., 27, 3008-3022 (2007). 17296732

Health Report

Examination Date / Room / Rack2008/05/13Room:4-5Rack:B


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cmahcytidine monophospho-N-acetylneuraminic acid hydroxylase13Cmah<tm1Ykoz>targeted mutation 1, Yasunori Kozutsumi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13


Donor DNAmouse Cmah genomic DNA, mouse PGK promoter, E.coli reomycin resistance gene
Research applicationMetabolism Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 27, 3008-3022 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use of the BIOLOGICAL RESOURCE only for publication of research papers.
DepositorYasunori Kozutsumi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data