Strain Data Sheet

RBRC02317

Information1

Image
BRC No.RBRC02317
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Pde10a2<tm1(tTA)Yok>
Former Common namePDE10A2-tTA
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Yokoi, Columbia University in 2002.
Strain descriptionPde10a2 tTA knock-in mice. A tTA gene was knocked into PDE10A2 gene locus, and the PDE10A2 gene is knocked out. The homozygotes and heterozygotes can be used as PDE10A2-knockout mice, and as a tool for the specific expression in striatal projection neurons, respectively.
Colony maintenance
ReferencesGenetics, 45, 123-128 (2007) 17299748
J. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack2022/02/14Room:3-CRack:L
2021/11/15Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pde10aphosphodiesterase 10A17Pde10a<tm1(tTA)Yok>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)17tTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain

Information2

Donor DNAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain), Phage P1 loxP site, mouse Pde10a2 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 27, 6948-6955 (2007).RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Suzuki E, Momiyama T.
M1 muscarinic acetylcholine receptor-mediated inhibition of GABA release from striatal medium spiny neurons onto cholinergic interneurons.
Eur J Neurosci (2020) 33270289