Strain Data Sheet

RBRC02308

Strain Information

Image
BRC No.RBRC02308
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cxcl14<tm1>
Former Common nameB6BRAKwt/flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Ryu-Ichiro Hata, Kanagawa Dental College in 2008.
Strain descriptionC57BL/6, Cxcl14(BRAK) gene exon2 was substituted with floxp neo gene
Colony maintenanceSibling Mating (Heterozygote x Heterozygote, Wild-type)
References
BRAK/CXCL14 expression suppresses tumor growth in vivo in human oral carcinoma cells.
Ozawa S, Kato Y, Komori R, Maehata Y, Kubota E, Hata R
Biochem. BIophys. Res. Commun., 348, 406-412 (2006). 16884687

Inflamm. Regen., 30, 536-541 (2010).

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cxcl14C-X-C motif chemokine ligand 1413Cxcl14targeted mutation 1, Takahiko Hara
loxPphage P1 loxP13loxP
loxPphage P1 loxP13loxP
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • perinatal lethality, incomplete penetrance(MP:0011090)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Cxcl14 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The first paper making use of the donated mouse must be collaboration with the DEPOSITOR.
    DepositorRyu-Ichiro Hata (Kanagawa Dental College)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data