RBRC02308

Information1

Image
BRC No.RBRC02308
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Cxcl14<tm1>
Former Common nameB6BRAKwt/flox
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strainC57BL/6
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Ryu-Ichiro Hata, Kanagawa Dental College in 2008.
Strain descriptionC57BL/6, Cxcl14(BRAK) gene exon2 was substituted with floxp neo gene
Colony maintenanceSibling Mating (Heterozygote x Heterozygote, Wild-type)
ReferencesBiochem. BIophys. Res. Commun., 348, 406-412 (2006). 16884687
Inflamm. Regen., 30, 536-541 (2010).

Health Report

Examination Date / Room / Rack2019/08/26Room:3-BRack:C
2019/06/17Room:3-BRack:C
2019/04/15Room:3-BRack:C
2019/02/18Room:3-BRack:C
2018/12/17Room:3-BRack:C
2018/10/15Room:3-BRack:C
2018/09/07Room:3-BRack:C
2018/08/20Room:3-BRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cxcl14chemokine (C-X-C motif) ligand 1413Cxcl14<tm1Tha>targeted mutation 1, Takahiko Hara

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP13loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAPhage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Cxcl14 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The first paper making use of the donated mouse must be collaboration with the DEPOSITOR.
DepositorRyu-Ichiro Hata (Kanagawa Dental College)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data