RBRC02303

Information1

Image
BRC No.RBRC02303
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Lyzs<tm1(cre)Ifo>
Former Common nameLysM-Cre knock-in mice (BALB/c)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Technical University of Munich, Irmgard Foester. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303).
Strain descriptionLyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303).
Colony maintenanceHomozygote x Homozygote [or Crossing to BALB/cAnN]
ReferencesTransgenic Res., 8, 265-277 (1999). 10621974

Health Report

Examination Date / Room / Rack2014/07/14Room:4-BRack:C
2014/05/19Room:4-BRack:C
2014/03/24Room:4-BRack:C
2014/01/20Room:4-BRack:C
2013/11/25Room:4-BRack:C
2013/09/24Room:4-BRack:C
2013/07/22Room:4-BRack:C
2013/05/20Room:4-BRack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Lyzslysozyme10Lyzs<tm1(cre)Ifo>targeted mutation 1, Irmgard Foerster

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site10Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase10cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)10nls

Information2

Donor DNAmouse lysozyme M genomic DNA, Phage P1 cre, Simian virus 40 Large T antigen nuclear localization signal (NLS), yeast FRT (flipase recombination target) site
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER. Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de.
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data