RBRC02300

Information1

Image
BRC No.RBRC02300
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pten<tm2Mak>
Former Common namePten-flox mouse (C57BL/6)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Tak W. Mak, Ontario Cancer Institute in 2001. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine. C57BL/6N background.
Strain descriptionPten is a lipid phosphatase associated with tumor suppression. This conditional knockout mice have a loxP-flanked Pten allele. Conditional Pten deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenancebackcross to C57BL/6 (Heterozygote x C57BL/6NTac)
ReferencesImmunity, 14, 523-534 (2001) 11371355

Health Report

Examination Date / Room / Rack2016/11/28Room:4-CRack:A
2016/09/26Room:4-CRack:A
2016/07/25Room:4-CRack:A
2016/05/30Room:4-CRack:A
2016/03/28Room:4-CRack:A
2016/01/25Room:4-CRack:A
2015/11/30Room:4-CRack:A
2015/09/28Room:4-CRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptenphosphatase and tensin homolog19Pten<tm2Mak>targeted mutation 2, Tak W Mak2310035O07Rik, A130070J02Rik, MMAC1, TEP1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Information2

Donor DNAPhage P1 loxP sites, mouse Pten genomic DNA
Research applicationCancer Research
Cell Biology Research
Cre/loxP system
General Purpose
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP

BRC mice in Publications

Renault-Mihara F, Mukaino M, Shinozaki M, Kumamaru H, Kawase S, Baudoux M, Ishibashi T, Kawabata S, Nishiyama Y, Sugai K, Yasutake K, Okada S, Nakamura M, Okano H.
Regulation of RhoA by STAT3 coordinates glial scar formation.
J. Cell Biol. 216(8) 2533-2550(2017) 28642362

Mayuko Kanayama, Kazuki Nakao, Shigeo Horie, Atsu Aiba.
Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase.
Prostate Int. (2018) 30140659