Strain Data Sheet


Strain Information

BRC No.RBRC02300
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pten<tm2Mak>
Former Common namePten-flox mouse (C57BL/6)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Tak W. Mak, Ontario Cancer Institute in 2001. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine. C57BL/6N background.
Strain descriptionPten is a lipid phosphatase associated with tumor suppression. This conditional knockout mice have a loxP-flanked Pten allele. Conditional Pten deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenancebackcross to C57BL/6 (Heterozygote x C57BL/6NTac)
ReferencesImmunity, 14, 523-534 (2001) 11371355

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ptenphosphatase and tensin homolog19Pten<tm2Mak>targeted mutation 2, Tak W Mak2310035O07Rik, A130070J02Rik, MMAC1, TEP1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP19loxP

Ordering Information

Donor DNAPhage P1 loxP sites, mouse Pten genomic DNA
Research applicationCancer Research
Cell Biology Research
Cre/loxP system
General Purpose
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail:
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Renault-Mihara F, Mukaino M, Shinozaki M, Kumamaru H, Kawase S, Baudoux M, Ishibashi T, Kawabata S, Nishiyama Y, Sugai K, Yasutake K, Okada S, Nakamura M, Okano H.
Regulation of RhoA by STAT3 coordinates glial scar formation.
J Cell Biol 216(8) 2533-2550(2017) 28642362

Kanayama M, Nakao K, Horie S, Aiba A.
Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase.
Prostate Int 6(3) 99-103(2018) 30140659