Strain Data Sheet
RBRC02300
Information1 | |
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| Image |  |
| BRC No. | RBRC02300 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129P2-Pten<tm2Mak> |
| Former Common name | Pten-flox mouse (C57BL/6) |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Dr. Tak W. Mak, Ontario Cancer Institute in 2001. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine. C57BL/6N background. |
| Strain description | Pten is a lipid phosphatase associated with tumor suppression. This conditional knockout mice have a loxP-flanked Pten allele. Conditional Pten deficient mice can be generated by crossing with tissue-specific Cre mice. |
| Colony maintenance | backcross to C57BL/6 (Heterozygote x C57BL/6NTac) |
| References | Immunity, 14, 523-534 (2001) 11371355 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ptenphosphatase and tensin homolog19Pten<tm2Mak>targeted mutation 2, Tak W Mak2310035O07Rik, A130070J02Rik, MMAC1, TEP1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP |
Information2 | |
|---|---|
| Donor DNA | Phage P1 loxP sites, mouse Pten genomic DNA |
| Research application | Cancer Research Cell Biology Research Cre/loxP system General Purpose |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca). |
| Depositor | Shigeo Koyasu (Keio University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Renault-Mihara F, Mukaino M, Shinozaki M, Kumamaru H, Kawase S, Baudoux M, Ishibashi T, Kawabata S, Nishiyama Y, Sugai K, Yasutake K, Okada S, Nakamura M, Okano H. Regulation of RhoA by STAT3 coordinates glial scar formation. J Cell Biol 216(8) 2533-2550(2017) 28642362 Kanayama M, Nakao K, Horie S, Aiba A. Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase. Prostate Int 6(3) 99-103(2018) 30140659 |