Strain Data Sheet


Strain Information

BRC No.RBRC02298
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Il3<tm1Glli>
Former Common nameIL-3 knock-out mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cAnNTac
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Glenn Dranoff, Dana-Farber Cancer Institute, Harvard Medical School in 1998. Mutant mice were backcrossed to C57BL/6 for 9 gnerations, and then to BALB/cAnNTac for 3 generations. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine.
Strain descriptionInterluekin-3 is a growth factor of hematopoieic progenitors that yields multipotential blast cells, mast cells, basophils,neutrophils, macrophages, eosinophils, erythrocytes, megakaryocytes, and dendritic cells. IL-3 null mutant mice show unimpaired hematopoiesis, but contact hypersensitivity reactions are compromised.
Colony maintenanceBackcross to BALB/c (Heterozygote x BALB/cAnNTac)
ReferencesBlood, 91, 778-783 (1998). 9446636

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Il3Interleukin 311Il3<tm1Glli>targeted mutation 1, Stephen J GalliBPA, Csfmu, HCGF, Il-3, MCGF, PSF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Amouse PGK polyA11poly A

Ordering Information

Donor DNAmouse Il3 genomic DNA, mouse Phosphoglycerate kinase-1(PGK) promoter, E. coli neo, mouse PGK polyA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Glenn Dranoff, MD, Harvard Medical School, Dana-Farber Cancer Institute E-mail:
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Bénard A, Mittelstädt A, Klösch B, Glanz K, Müller J, Schoen J, Nüse B, Brunner M, Naschberger E, Stürzl M, Mattner J, Muñoz LE, Sohn K, Grützmann R, Weber GF.
IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils.
Cell Rep 42(6) 112637(2023) 37300834

Motomura Y, Morita H, Moro K, Nakae S, Artis D, Endo TA, Kuroki Y, Ohara O, Koyasu S, Kubo M.
Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation.
Immunity 40(5) 758-71(2014) 24837103

Balam S, Schiechl-Brachner G, Buchtler S, Halbritter D, Schmidbauer K, Talke Y, Neumayer S, Salewski JN, Winter F, Karasuyama H, Yamanishi Y, Renner K, Geissler EK, Mack M.
IL-3 Triggers Chronic Rejection of Cardiac Allografts by Activation of Infiltrating Basophils.
J Immunol 202(12) 3514-3523(2019) 31068389

Leyva-Castillo JM, Hener P, Michea P, Karasuyama H, Chan S, Soumelis V, Li M.
Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade.
Nat Commun 4 2847(2013) 24284909

Weber GF, Chousterman BG, He S, Fenn AM, Nairz M, Anzai A, Brenner T, Uhle F, Iwamoto Y, Robbins CS, Noiret L, Maier SL, Zönnchen T, Rahbari NN, Schölch S, Klotzsche-von Ameln A, Chavakis T, Weitz J, Hofer S, Weigand MA, Nahrendorf M, Weissleder R, Swirski FK.
Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis.
Science 347(6227) 1260-5(2015) 25766237

Julia Ko¨lle, Theodor Zimmermann, Alexander Kiefer, Ralf J. Rieker, Paraskevi Xepapadaki, Sebastian Zundler, Nikolaos G. Papadopoulos, Susetta Finotto
Targeted deletion of Interleukin-3 results in asthma exacerbations
iScience 104440(2022) 35707726