RBRC02298

Information1

Image
BRC No.RBRC02298
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC.Cg-Il3<tm1Glli>
Former Common nameIL-3 knock-out mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainBALB/cAnNTac
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Dr. Glenn Dranoff, Dana-Farber Cancer Institute, Harvard Medical School in 1998. Mutant mice were backcrossed to C57BL/6 for 9 gnerations, and then to BALB/cAnNTac for 3 generations. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine.
Strain descriptionInterluekin-3 is a growth factor of hematopoieic progenitors that yields multipotential blast cells, mast cells, basophils,neutrophils, macrophages, eosinophils, erythrocytes, megakaryocytes, and dendritic cells. IL-3 null mutant mice show unimpaired hematopoiesis, but contact hypersensitivity reactions are compromised.
Colony maintenanceBackcross to BALB/c (Heterozygote x BALB/cAnNTac)
ReferencesBlood, 91, 778-783 (1998). 9446636

Health Report

Examination Date / Room / Rack2015/01/19Room:4-BRack:F
2014/11/17Room:4-BRack:F
2014/09/16Room:4-BRack:F
2014/07/14Room:4-BRack:F
2014/05/19Room:4-BRack:F
2014/03/24Room:4-BRack:F
2014/01/20Room:4-BRack:F
2013/11/25Room:4-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Il3Interleukin 311Il3<tm1Glli>targeted mutation 1, Stephen J Galli

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly Amouse PGK poly A11poly A

Information2

Donor DNAmouse Il3 genomic DNA, mouse Phosphoglycerate kinase-1(PGK) promoter, E. coli neo, mouse PGK polyA
Research applicationImmunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Glenn Dranoff, MD, Harvard Medical School, Dana-Farber Cancer Institute E-mail: glenn_dranoff@dfci.harvard.edu).
DepositorShigeo Koyasu (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

Motomura Y, Morita H, Moro K, Nakae S, Artis D, Endo TA, Kuroki Y, Ohara O, Koyasu S, Kubo M.
Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation.
Immunity 40(5) 758-71(2014) 24837103

Leyva-Castillo JM, Hener P, Michea P, Karasuyama H, Chan S, Soumelis V, Li M.
Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade.
Nat Commun 4 2847(2013) 24284909

Weber GF, Chousterman BG, He S, Fenn AM, Nairz M, Anzai A, Brenner T, Uhle F, Iwamoto Y, Robbins CS, Noiret L, Maier SL, Zönchen T, Rahbari NN, Schöch S, Klotzsche-von Ameln A, Chavakis T, Weitz J, Hofer S, Weigand MA, Nahrendorf M, Weissleder R, Swirski FK.
Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis.
Science 347(6227) 1260-5.(2015) 25766237