RBRC02295

Information1

Image
BRC No.RBRC02295
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy
Former Common nameCas-ex2<flox/flox>-Tg(BLG-Cre)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain descriptionCasp130 (Bcar1) gene exon 2 floxed and BLG (beta lactoglobulin)-cre double mutant mice. Viable and fertile, but the reproductive performance is not so good.
Colony maintenanceKO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]
ReferencesHepatology, 52(3), 1089-1099 (2010). 20623582

Health Report

Examination Date / Room / Rack2014/02/03Room:3-4Rack:D
2013/12/02Room:3-4Rack:D
2013/10/07Room:3-4Rack:D
2013/08/05Room:3-4Rack:D
2013/06/03Room:3-4Rack:D
2013/04/01Room:3-4Rack:D
2013/02/04Room:3-4Rack:D
2012/12/03Room:3-4Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Bcar1breast cancer anti-estrogen resistance 18Bcar1<tm1.2Homy>targeted mutation 1.2, Homy

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcresheep BLG promoter

Information2

Donor DNAphage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA, sheep BLG genomic DNA, phage P1 Cre-recombinase
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data