Strain Data Sheet

RBRC02295

Strain Information

Image
BRC No.RBRC02295
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy
Former Common nameCas-ex2<flox/flox>-Tg(BLG-Cre)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain descriptionCasp130 (Bcar1) gene exon 2 floxed and BLG (beta lactoglobulin)-cre double mutant mice. Viable and fertile, but the reproductive performance is not so good.
Colony maintenanceKO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]
References
p130Cas, Crk-associated substrate plays essential roles in liver development by regulating sinusoidal endothelial cell fenestration.
Tazaki T, Sasaki T, Uto K, Yamasaki N, Tashiro S, Sakai R, Tanaka M, Oda H, Honda Z, Honda H
Hepatology, 52(3), 1089-1099 (2010). 20623582

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcar1breast cancer anti-estrogen resistance 18Bcar1targeted mutation 1.2, Homy
Frtyeast FRT (flippase recombination target) site8Frt
loxPphage P1 loxP8loxP
loxPphage P1 loxP8loxP
crePhage P1 Cre recombinaseUNcresheep BLG promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA, sheep BLG genomic DNA, phage P1 Cre-recombinase
    Research applicationCell Biology Research
    Cre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data