RBRC02291

Information1

Image
BRC No.RBRC02291
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(tetO-spH)CYoko
Former Common nameTetO-synaptopHluorin
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Kyoto University Graduate School of Medicine in 2005.
Strain descriptionThis transgenic mice express the GFP in the neuron axon terminal under the control of the tetracycline operator sequence.
Colony maintenanceHemizygote x Wild-type (C57BL/6CrSlc)
ReferencesJ. Neurosci., 27, 6948-6955 (2007) 17596443

Health Report

Examination Date / Room / Rack2010/12/13Room:3-CRack:I
2010/10/18Room:3-CRack:I
2010/08/23Room:3-CRack:I
2010/06/21Room:3-CRack:I
2010/04/19Room:3-CRack:I
2010/02/22Room:3-CRack:I
2009/12/14Room:3-CRack:I
2009/10/19Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Vamp2vesicle-associated membrane protein 2UNVamp2E. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin intron/polyA signalUNHBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
pHluorinpH-sensitive green fluorescent proteins, S147E, N149L, I161T, V163A, N164I, K166Q, I167V, R168H, S202H and L231H. (Jellyfish)UNpHluorin

Information2

Donor DNAE. coli tetO, CMV minimal promoter, mouse vesicular membrane protein Vamp2 cDNA, Jellyfish pH-sensitive mutants GFP cDNA (pHluorin cDNA(Miesenbck, G., De Angelis, D.A., Rothman, J. E. Nature, 394, 192-195, 1998).), rabbit beta-globin intron/polyA signal
Research applicationFluorescent Proteins/lacZ System
Neurobiology Research
Tet system
General Purpose
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J.Neurosci., 27, 6948-6955 (2007).The RECIPIENT may only use the BIOLOGICAL RESOURCE only for non-commercial academic research purpose.
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data