Strain Data Sheet


Strain Information

BRC No.RBRC02290
SpeciesMus musculus
Strain nameSTOCK Tg(Nanog-GFP,Puro)1Yam
Former Common name
H-2 Haplotype
ES Cell lineRF8 [129S4/SvJae]
Background strain
Strain developmentDeveloped by Dr. Shinya Yamanaka, Institute for Frontier Medical Sciences, Kyoto University in 2003. The construct was injected into RF8 ES cells derived from 129S4/SvJae. 129, C57BL/6 and DBA/2 mixed background.
Strain descriptionNanog-GFP transgenic mice. Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). This Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror) cassette into the 5' untranslated region of a bacterial artificial chromosome (BAC) containing the mouse Nanog gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency. Tg homozygous and hemizygous mice were viable and fertile. Male-biased sex ratios was observed (199 females : 261 males)(details unknown).
Colony maintenanceCarrier x Carrier (Homozygote x Homozygote)
ReferencesNature, 448, 313-317 (2007) 17554338

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNNanog promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)UNinternal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)UNpuro

Ordering Information

Donor DNAJellyfish EGFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (IRES), S. alboniger puromycin resistance gene, bovine growth hormone polyadenylation signal, yeast FRT (flipase recombination target) site, mouse genomic DNA from RP23-117GFPNF
Research applicationCell Biology Research
Developmental Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions
DepositorShinya Yamanaka (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: (Japanese/ English)
  3. Distribution of the iPS cells (Japanese/ English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Dec 2007
Mouse of the Month Oct 2012

BRC mice in Publications

Nagamatsu G, Kosaka T, Saito S, Takubo K, Akiyama H, Sudo T, Horimoto K, Oya M, Suda T.
Tracing the conversion process from primordial germ cells to pluripotent stem cells in mice.
Biol Reprod 86(6) 182(2012) 22423052

Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S.
Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells.
Cell Stem Cell 14(2) 217-27(2014) 24506885

Nukaya D, Minami K, Hoshikawa R, Yokoi N, Seino S.
Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas.
Genes Cells 20(5) 367-81(2015) 25727848

Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T.
A germ cell-specific gene, Prmt5, works in somatic cell reprogramming.
J Biol Chem 286(12) 10641-8(2011) 21270127

Jincho Y, Araki R, Hoki Y, Tamura C, Nakamura M, Ando S, Kasama Y, Abe M.
Generation of genome integration-free induced pluripotent stem cells from fibroblasts of C57BL/6 mice without c-Myc transduction.
J Biol Chem 285(34) 26384-9(2010) 20554535

Araki R, Hoki Y, Suga T, Obara C, Sunayama M, Imadome K, Fujita M, Kamimura S, Nakamura M, Wakayama S, Nagy A, Wakayama T, Abe M.
Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency.
Nat Commun 11(1) 197(2020) 31924765

Koga M, Matsuda M, Kawamura T, Sogo T, Shigeno A, Nishida E, Ebisuya M.
Foxd1 is a mediator and indicator of the cell reprogramming process.
Nat Commun 5 3197(2014) 24496101

Okita K, Hong H, Takahashi K, Yamanaka S.
Generation of mouse-induced pluripotent stem cells with plasmid vectors.
Nat Protoc 5(3) 418-28(2010) 20203661

Cavallari C, Fonsato V, Herrera MB, Bruno S, Tetta C, Camussi G.
Role of Lefty in the anti tumor activity of human adult liver stem cells.
Oncogene 32(7) 819-26(2013) 22469982

Iseki H, Nakachi Y, Hishida T, Yamashita-Sugahara Y, Hirasaki M, Ueda A, Tanimoto Y, Iijima S, Sugiyama F, Yagami K, Takahashi S, Okuda A, Okazaki Y.
Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells.
Stem Cells 34(2) 322-33(2016) 26523946

Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando S, Sugiura M, Yoshida MA, Kasama Y, Abe M.
Crucial role of c-Myc in the generation of induced pluripotent stem cells.
Stem Cells 29(9) 1362-70(2011) 21732496

Araki R, Jincho Y, Hoki Y, Nakamura M, Tamura C, Ando S, Kasama Y, Abe M.
Conversion of ancestral fibroblasts to induced pluripotent stem cells.
Stem Cells 28(2) 213-20(2010) 20020427

Swistowski A, Peng J, Liu Q, Mali P, Rao MS, Cheng L, Zeng X.
Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions.
Stem Cells 28(10) 1893-904(2010) 20715183

Borisova E, Nishimura K, An Y, Takami M, Li J, Song D, Matsuo-Takasaki M, Luijkx D, Aizawa S, Kuno A, Sugihara E, Sato TA, Yumoto F, Terada T, Hisatake K, Hayashi Y.
Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency.
iScience 25(1) 103525(2022) 35106457