Strain Data Sheet

RBRC02280

Information1

Image
BRC No.RBRC02280
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Ephb6<tm1Tom>
Former Common nameEphB6 knock-out mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Toshimitsu Matsui, Kobe University in 2002.
Strain descriptionB6.129S4-Ephb6<tm1Tom>. EphB6, Eph receptor B6, is a member of the largest family of receptor protein kinases. Homozygous mutant mice developed normally and fertile, but T cell responses in them were compromised. This strain is useful for elucidating the EphB6 gene function.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
ReferencesBiochem. Biophys. Res. Commun., 298, 87-94 (2002). 12379224

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ephb6Eph receptor B66Ephb6<tm1Tom>targeted mutation 1, Toshimitsu MatsuiCekl, Mep

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Ephb6 genomic DNA
Research applicationCancer Research
Cell Biology Research
Cre/loxP system
Immunology and Inflammation Research
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 298, 87-94 (2002).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Shimoyama M. et al. Biochem Biophys Res Commun 298; 87-94: 2002
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use or publication with the results from the use of the BIOLOGICAL RESOURCE. However, it may not be a collaboration if the research interest is out of field of the DEPOSITOR.
DepositorToshimitsu Matsui (Kobe University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data