Strain Data Sheet

RBRC02280

Strain Information

Image
BRC No.RBRC02280
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Ephb6<tm1Tom>
Former Common nameEphB6 knock-out mouse
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Toshimitsu Matsui, Kobe University in 2002.
Strain descriptionB6.129S4-Ephb6<tm1Tom>. EphB6, Eph receptor B6, is a member of the largest family of receptor protein kinases. Homozygous mutant mice developed normally and fertile, but T cell responses in them were compromised. This strain is useful for elucidating the EphB6 gene function.
Colony maintenanceSibling Mating (Homozygote x Homozygote)
References
Developmental expression of EphB6 in the thymus: lessons from EphB6 knockout mice.
Shimoyama M, Matsuoka H, Nagata A, Iwata N, Tamekane A, Okamura A, Gomyo H, Ito M, Jishage K, Kamada N, Suzuki H, Tetsuo Noda T, Matsui T
Biochem. Biophys. Res. Commun., 298, 87-94 (2002). 12379224

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ephb6Eph receptor B66Ephb6targeted mutation 1, Toshimitsu Matsui
loxPphage P1 loxP6loxP
loxPphage P1 loxP6loxP
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • hematopoietic system phenotype(MP:0005397)

  • immune system phenotype(MP:0005387)

  • neoplasm(MP:0002006)

  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, mouse Ephb6 genomic DNA
    Research applicationCancer Research
    Cell Biology Research
    Cre/loxP system
    Immunology and Inflammation Research
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 298, 87-94 (2002).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Shimoyama M. et al. Biochem Biophys Res Commun 298; 87-94: 2002
    The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use or publication with the results from the use of the BIOLOGICAL RESOURCE. However, it may not be a collaboration if the research interest is out of field of the DEPOSITOR.
    DepositorToshimitsu Matsui (Kobe University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data