Strain Data Sheet

RBRC02277

Strain Information

Image
BRC No.RBRC02277
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Nuak1<tm1Sia>/SiaRbrc
Former Common nameOmphk(lacZ) (Acc. No. CDB0029K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
A new serine/threonine protein kinase, Omphk1, essential to ventral body wall formation.
Hirano M, Kiyonari H, Inoue A, Furushima K, Murata T, Suda Y, Aizawa S
Dev. Dyn., 235, 2229-2237 (2006). 16715502

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site10Frt
Frtyeast FRT (flippase recombination target) site10Frt
HBB2rabbit beta-globin polyA10HBB2
Nuak1NUAK family, SNF1-like kinase, 110Nuak1targeted mutation 1, Shinichi Aizawa
lacZbeta-galactosidase (E. coli)10
lox71mutant lox sequence10lox71
loxPphage P1 loxP10loxP
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • herniated abdominal wall(MP:0000757)

  • nervous system phenotype(MP:0003631)

  • omphalocele(MP:0003052)

  • perinatal lethality, complete penetrance(MP:0011089)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage lox71 site, E. coli lacZ, rabbit beta-globin polyA, yeast frt sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neo, P1 phage loxP site, mouse Pgk1 polyA, mouse Nuak1 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0029K and the BIOLOGICAL RESOURCE is requested (https://large.riken.jp/distribution/mutant-list.html). (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Developmental Dynamics 235, 2229-2237 (2006). (3) Please inform the DEVELOPER (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data