Strain Data Sheet

RBRC02271

Strain Information

Image
BRC No.RBRC02271
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Otx2<tm4.1Sia>/SiaRbrc
Former Common nameOtx2 conditional KO flox (Acc. No. CDB0013K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Center for Developmental Biology. TT2 ES cells derived from (C57BL/6 x CBA)F1 were used for generation of the mice. A frt-flanked neo cassette was removed by crossing with flp mice.
Strain descriptionOtx2 gene floxed mice. The promoter and first exon were flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
References
Otx2 is required to respond to signals from anterior neural ridge for forebrain specification.
Tian E, Kimura C, Takeda N, Aizawa S, Matsuo I
Development. Biol., 242, 204-223 (2005) 11820816

Health Report

Examination Date / Room / Rack2023/09/11Room:3-6Rack:C
2023/06/13Room:3-6Rack:C
2023/03/13Room:3-6Rack:C
2022/12/12Room:3-6Rack:C

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site14Frt
  • pituitary hormone deficiency, combined, 6(MedGEN)

  • syndromic microphthalmia type 5(MedGEN)
    • Otx2orthodenticle homeobox 214Otx2targeted mutation 4.1, Shinichi Aizawa
  • pituitary hormone deficiency, combined, 6(MedGEN)

  • syndromic microphthalmia type 5(MedGEN)
    • loxPphage P1 loxP14loxP
  • pituitary hormone deficiency, combined, 6(MedGEN)

  • syndromic microphthalmia type 5(MedGEN)
    • loxPphage P1 loxP14loxP
  • pituitary hormone deficiency, combined, 6(MedGEN)

  • syndromic microphthalmia type 5(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, yeast frt site, mouse Otx2 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0013K and the BIOLOGICAL RESOURCE is requested (https://large.riken.jp/distribution/mutant-list.html). (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Developmental Biology 242, 204-223 (2002). (3) Please inform the DEVELOPER (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data