Strain Data Sheet

RBRC02271

Information1

Image
BRC No.RBRC02271
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Otx2<tm4.1Sia>/SiaRbrc
Former Common nameOtx2 conditional KO flox (Acc. No. CDB0013K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Center for Developmental Biology. TT2 ES cells derived from (C57BL/6 x CBA)F1 were used for generation of the mice. A frt-flanked neo cassette was removed by crossing with flp mice.
Strain descriptionOtx2 gene floxed mice. The promoter and first exon were flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrlCrlj]
ReferencesDevelopment. Biol., 242, 204-223 (2005) 11820816

Health Report

Examination Date / Room / Rack2023/03/13Room:3-6Rack:C
2022/12/12Room:3-6Rack:C
2022/09/12Room:3-6Rack:C
2022/06/13Room:3-6Rack:C
2022/03/14Room:3-6Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Otx2orthodenticle homolog 2 (Drosophila)14Otx2<tm4.1Sia>targeted mutation 4.1, Shinichi AizawaE130306E05Rik

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Information2

Donor DNAP1 phage loxP sites, yeast frt site, mouse Otx2 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0013K and the BIOLOGICAL RESOURCE is requested (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html). (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Developmental Biology 242, 204-223 (2002). (3) Please inform the DEVELOPER (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data