Strain Data Sheet

RBRC02269

Strain Information

Image
BRC No.RBRC02269
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Sgcg<tm1Oza>
Former Common nameGSG-KO (C57BL/6J), B6-Sgcg KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Toshikuni Sasaoka, National Institutes of Natural Sciences (National Institute of Neuroscience, National Center of Neurology and Psychiatry).
Strain descriptionGamma-sarcoglycan (Sgcg) gene knockout mice. Sgcg is a transmembrane, dystrophin-associated protein expressed in skeletal and cardiac muscle. Homozygous mutant mice show skeletal and cardiac muscle degeneration.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Pathological analysis of muscle hypertrophy and degeneration in muscular dystrophy in gamma-sarcoglycan-deficient mice.
Sasaoka T, Imamura M, Araishi K, Noguchi S, Mizuno Y, Takagoshi N, Hama H, Wakabayashi-Takai E, Yoshimoto-Matsuda Y, Nonaka I, Kaneko K, Yoshida M, Ozawa E
Neuromuscul. Disord., 13, 193-206 (2003). 12609501

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sgcgsarcoglycan, gamma (dystrophin-associated glycoprotein)14Sgcgtargeted mutation 1, Eijiro Ozawa
  • autosomal recessive limb-girdle muscular dystrophy type 2C(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)
  • autosomal recessive limb-girdle muscular dystrophy type 2C(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal diaphragm morphology(MP:0002279)

  • abnormal muscle regeneration(MP:0000750)

  • abnormal tibialis anterior morphology(MP:0003083)

  • cardiac fibrosis(MP:0003141)

  • dystrophic muscle(MP:0000752)
    • more 10 phenotypes
  • increased circulating creatine kinase level(MP:0010090)

  • increased skeletal muscle fiber size(MP:0009399)

  • increased skeletal muscle mass(MP:0004818)

  • muscle degeneration(MP:0000749)

  • myocardium necrosis(MP:0006085)

  • premature death(MP:0002083)

  • progressive muscle weakness(MP:0000748)

  • skeletal muscle hypertrophy(MP:0009461)

  • skeletal muscle interstitial fibrosis(MP:0003851)

  • skeletal muscle necrosis(MP:0003852)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E coli neo, mouse gamma-sarcoglycan (GSG) genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuromuscul. Disord., 13, 193-206 (2003).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Toshikuni Sasaoka is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data