Strain Data Sheet

RBRC02268

Strain Information

Image
BRC No.RBRC02268
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Sgcb<tm1Oza>
Former Common nameBSG-KO (C57BL/6J), B6-Sgcb KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Toshikuni Sasaoka, National Institutes of Natural Sciences (National Institute of Neuroscience, National Center of Neurology and Psychiatry).
Strain descriptionBeta-sarcoglycan (Sgcb) gene knockout mice. Sgcb is an integral component of the dystrophin-glycoprotein complex. Homozygous mutant mice show progressive muscular dystrophy with extensive degeneration and regeneration.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Loss of the sarcoglycan complex and sarcospan leads to muscular dystrophy in beta-sarcoglycan-deficient mice.
Araishi K, Sasaoka T, Imamura M, Noguchi S, Hama H, Wakabayashi E, Yoshida M, Hori T, Ozawa E
Hum Mol Genet. 1999 8(9):1589-98. 10441321

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sgcbsarcoglycan, beta (dystrophin-associated glycoprotein)5Sgcbtargeted mutation 1, Eijiro Ozawa
  • autosomal recessive limb-girdle muscular dystrophy type 2E(MedGEN)
    • neoneomycin resistance gene (E. coli)5
  • autosomal recessive limb-girdle muscular dystrophy type 2E(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal skeletal muscle morphology(MP:0000759)

  • cardiac fibrosis(MP:0003141)

  • centrally nucleated skeletal muscle fibers(MP:0009404)

  • dystrophic muscle(MP:0000752)

  • increased body size(MP:0001264)
    • more 6 phenotypes
  • increased body weight(MP:0001260)

  • increased gastrocnemius weight(MP:0009421)

  • increased tibialis anterior weight(MP:0009427)

  • myositis(MP:0004510)

  • skeletal muscle fiber degeneration(MP:0009412)

  • skeletal muscle fiber necrosis(MP:0009414)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE coli neo, mouse beta-sarcoglycan (BSG) genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hum. Mol. Genet., 8, 1589-1598 (1999). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Toshikuni Sasaoka is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data