Strain Data Sheet

RBRC02260

update : 2024-08-20

Strain Information
Image
BRC No.	RBRC02260
Type	Targeted Mutation
Species	Mus musculus
Strain name	B6.129S-Trp53<tm2Mok>
Former Common name	p53KO, PFZ, PFZ632, B6-p53 KO
H-2 Haplotype
ES Cell line	CCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain	C57BL/6JJcl
Appearance	black [a/a B/B C/C]
Strain development	Developed by Motoya Katsuki, National Institutes of Natural Sciences (Medical Institute of Bioregulation, Kyushu University).
Strain description	p53 (Trp53) gene knockout mice (lacZ reporter).
Colony maintenance	Heterozygote x Wild-type [C57BL/6JJcl]
References	Gene replacement of the p53 gene with the lacZ gene in mouse embryonic stem cells and mice by using two steps of homologous recombination. Gondo Y, Nakamura K, Nakao K, Sasaoka T, Ito K, Kimura M, Katsuki M Biochem. Biophys. Res. Commun., 202, 830-837 (1994). 8048955

Health Report
Examination Date / Room / Rack

Gene
Gene Symbol	Gene Name	Chr.	Allele Symbol	Allele Name	Common Names	Promoter	Diseases Related to This Gene
Trp53	transformation related protein 53	11	Trp53	targeted mutation 2, Motoya Katsuki			adrenal cortex carcinoma(DisGeNET) adrenocortical carcinoma, hereditary(MedGEN) B-cell chronic lymphocytic leukemia(DisGeNET) more 14 Diseases basal cell carcinoma, susceptibility to, 7(MedGEN) bone marrow failure syndrome 5(MedGEN) bone osteosarcoma(MedGEN) breast carcinoma(DisGeNET) choroid plexus papilloma(MedGEN) colorectal cancer(MedGEN) exocrine pancreatic carcinoma(MedGEN) glioma susceptibility 1(MedGEN) glioma(DisGeNET) hepatocellular carcinoma(MedGEN) hereditary breast carcinoma(MedGEN) Li-Fraumeni syndrome(DisGeNET) nasopharyngeal carcinoma(MedGEN) obsolete Li-Fraumeni syndrome 1(MedGEN)
lacZ	beta-galactosidase (E. coli)	11					adrenal cortex carcinoma(DisGeNET) adrenocortical carcinoma, hereditary(MedGEN) B-cell chronic lymphocytic leukemia(DisGeNET) more 14 Diseases basal cell carcinoma, susceptibility to, 7(MedGEN) bone marrow failure syndrome 5(MedGEN) bone osteosarcoma(MedGEN) breast carcinoma(DisGeNET) choroid plexus papilloma(MedGEN) colorectal cancer(MedGEN) exocrine pancreatic carcinoma(MedGEN) glioma susceptibility 1(MedGEN) glioma(DisGeNET) hepatocellular carcinoma(MedGEN) hereditary breast carcinoma(MedGEN) Li-Fraumeni syndrome(DisGeNET) nasopharyngeal carcinoma(MedGEN) obsolete Li-Fraumeni syndrome 1(MedGEN)

Phenotype
Annotation by Mammalian phenotyhpe ontology	decreased cellular sensitivity to ultraviolet irradiation(MP:0008411) increased tumor incidence(MP:0002020)
Detailed phenotype data

Ordering Information
Donor DNA	E coli lacZ (beta galactosidase), mouse p53 genomic DNA
Research application	Cancer Research Cell Biology Research Fluorescent Proteins/lacZ System Immunology and Inflammation Research Mouse Models for Human Disease
Specific Term and Conditions	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 202, 830-837 (1994). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Depositor	Toshikuni Sasaoka (Niigata University)
Strain Status	Frozen embryos Frozen sperm
Strain Availability	Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month)
Additional Info.	Necessary documents for ordering: Order form (Japanese / English) Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English) Genotyping protocol -PCR-

BRC mice in Publications

No Data