Strain Data Sheet

RBRC02259

Strain Information

Image
BRC No.RBRC02259
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Kras<tm1Mok>
Former Common nameK-ras KO, B6-Kras KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionKras gene knockout mice. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
K-ras is essential for the development of the mouse embryo.
Koera K, Nakamura K, Nakao K, Miyoshi J, Toyoshima K, Hatta T, Otani H, Aiba A, Katsuki M
Oncogene, 15, 1151-1159 (1997). 9294608

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
KrasKirsten rat sarcoma viral oncogene homolog6Krastargeted mutation 1, Motoya Katsuki
  • acute myeloid leukemia(MedGEN)

  • arteriovenous malformations of the brain(MedGEN)

  • autoimmune lymphoproliferative syndrome type 4(MedGEN)
    • more 12 Diseases
  • cardiofaciocutaneous syndrome 2(MedGEN)

  • cardiofaciocutaneous syndrome(DisGeNET)

  • exocrine pancreatic carcinoma(MedGEN)

  • gastric cancer(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • juvenile myelomonocytic leukemia(DisGeNET)

  • linear nevus sebaceous syndrome(MedGEN)

  • lung cancer(MedGEN)

  • Noonan syndrome 3(DisGeNET, MedGEN)

  • Noonan syndrome(DisGeNET)

  • Toriello-Lacassie-Droste syndrome(MedGEN)

  • urinary bladder cancer(MedGEN)
    • neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • acute myeloid leukemia(MedGEN)

  • arteriovenous malformations of the brain(MedGEN)

  • autoimmune lymphoproliferative syndrome type 4(MedGEN)
    • more 12 Diseases
  • cardiofaciocutaneous syndrome 2(MedGEN)

  • cardiofaciocutaneous syndrome(DisGeNET)

  • exocrine pancreatic carcinoma(MedGEN)

  • gastric cancer(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • juvenile myelomonocytic leukemia(DisGeNET)

  • linear nevus sebaceous syndrome(MedGEN)

  • lung cancer(MedGEN)

  • Noonan syndrome 3(DisGeNET, MedGEN)

  • Noonan syndrome(DisGeNET)

  • Toriello-Lacassie-Droste syndrome(MedGEN)

  • urinary bladder cancer(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal fetal cardiomyocyte proliferation(MP:0003567)

  • abnormal heart morphology(MP:0000266)

  • abnormal lymphatic vessel morphology(MP:0001879)

  • decreased embryo size(MP:0001698)

  • embryonic growth retardation(MP:0003984)
    • more 6 phenotypes
  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • increased neuron apoptosis(MP:0003203)

  • no abnormal phenotype detected(MP:0002169)

  • small heart(MP:0002188)

  • thin myocardium(MP:0002652)

  • thin ventricular wall(MP:0000280)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse K-ras genomic DNA
    Research applicationCancer Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 15, 1151-1159 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data