Strain Data Sheet

RBRC02259

Information1

Image
BRC No.RBRC02259
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Kras<tm1Mok>
Former Common nameK-ras KO, B6-Kras KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionKras gene knockout mice. Homozygous mutant mice are embryonic lethal.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesOncogene, 15, 1151-1159 (1997). 9294608

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Krasv-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog6Kras<tm1Mok>targeted mutation 1, Motoya KatsukiK-ras, Ki-ras, Kras-2, Kras2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse K-ras genomic DNA
Research applicationCancer Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Oncogene, 15, 1151-1159 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorToshikuni Sasaoka (Niigata University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data