Strain Data Sheet

RBRC02256

Strain Information

Image
BRC No.RBRC02256
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S-Grin2a<tm1Nak>
Former Common nameNR2A KO
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeveloped by Motoya Katsuki, National Institutes of Natural Sciences (Institute of Medical Science, University of Tokyo).
Strain descriptionNR2A (Grin2a) gene knockout mice. Homozygous mutant mice are viable and fertile.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Motor discoordination results from combined gene disruption of the NMDA receptor NR2A and NR2C subunits, but not from single disruption of the NR2A or NR2C subunit.
H Kadotani, T Hirano, M Masugi, K Nakamura, K Nakao, M Katsuki, S Nakanishi
J. Neurosci., 16, 7859-7867 (1996). 8987814

Health Report

Examination Date / Room / Rack2026/03/09Room:4-BRack:ESentinel mouse program
2026/01/05Room:4-BRack:ESentinel mouse program
2025/10/20Room:4-BRack:ESentinel mouse program
2025/08/04Room:4-BRack:ESentinel mouse program
2025/05/26Room:4-BRack:ESentinel mouse program
2025/02/25Room:4-BRack:ESentinel mouse program
2024/11/25Room:4-BRack:ESentinel mouse program
2024/08/26Room:4-BRack:ESentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Grin2a
MGI:95820		glutamate receptor, ionotropic, NMDA2A (epsilon 1)16Grin2a<tm1Nak>
MGI:1928506		targeted mutation 1, Shigetada Nakanishi
  • Landau-Kleffner syndrome(MedGEN)
    • neo neomycin resistance gene (E. coli)16 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal excitatory postsynaptic currents (MP:0002910)

  • abnormal miniature excitatory postsynaptic currents (MP:0004753)

  • decreased vertical activity (MP:0002757)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E coli neo, mouse NMDAR2A genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Neurosci., 16, 7859-7867 (1996). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorToshikuni Sasaoka (Niigata University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Hosseini H, Evans-Martin S, Jones KS.
    Global Grin2a loss causes divergent impairments to PV+ and SST+ interneurons and alters gamma oscillations in prefrontal cortex.
    Neurobiol Dis 212 106977(2025) 40436282
    Perzel Mandell KA, Simmons SK, Nadig A, Farsi Z, Huang WC, Aryal S, Kwon MJ, Song B, Brenner K, Shepard N, Nicolella AA, O'Brien LD, Lichtman AH, Levin JZ, Sheng M.
    Meta-analysis of the brain transcriptomes of multiple genetic mouse models of schizophrenia highlights dysregulation in striatum and thalamus.
    Transl Psychiatry 15(1) 345(2025) 41022686
    Farsi Z, Nicolella A, Simmons SK, Aryal S, Shepard N, Brenner K, Lin S, Herzog L, Moran SP, Stalnaker KJ, Shin W, Gazestani V, Song BJ, Bonanno K, Keshishian H, Carr SA, Pan JQ, Macosko EZ, Datta SR, Dejanovic B, Kim E, Levin JZ, Sheng M.
    Brain-region-specific changes in neurons and glia and dysregulation of dopamine signaling in Grin2a mutant mice.
    Neuron 111(21) 3378-3396.e9(2023) 37657442
    Herzog LE, Wang L, Yu E, Choi S, Farsi Z, Song BJ, Pan JQ, Sheng M.
    Mouse mutants in schizophrenia risk genes GRIN2A and AKAP11 show EEG abnormalities in common with schizophrenia patients.
    Transl Psychiatry 13(1) 92(2023) 36914641