Strain Data Sheet

RBRC02253

Strain Information

Image
BRC No.RBRC02253
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(KRT5-Dsg3/EGFP)1
Former Common namemDsg3EGFP Tg, C57BL/6, C57BL/6-Tg(hK5-mDsg3/EGFP)1
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Drs. Kouji Nishifuji anf Masayuki Amagai, Keio University School of Medicine in 2006.
Strain descriptionThis transgenic mouse shows the expression of the fusion protein to fuse GFP at the C terminus of full-length mouse desmoglein 3 (Dsg3), at the stratified squamous epithelium under the control of human K5 promoter. Desmoglein (Dsg) is a cadhelin type adhesive molecule to express in the desmosome of stratified squamous epithelium. It is also known as a self antigen of the pemphigus vulgaris which is one of a autoimmune bullous disease. This mouse is useful for the study of the cell adhesion function of the desmosome and pathology of autoimmune bullous disease.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Dsg3desmoglein 3UNDsg3human Keratin 5 promoter
  • blistering, acantholytic, of oral and laryngeal mucosa(MedGEN)
    • EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
  • blistering, acantholytic, of oral and laryngeal mucosa(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman kerain 5 promoter, mouse desmoglein 3 cDNA, Jellyfish Green Fluorescent Protein (EGFP) cDNA
    Research applicationCell Biology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorMasayuki Amagai (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data