Strain Data Sheet

RBRC02252

Strain Information

Image
BRC No.RBRC02252
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(KRT5-DsRed2/KRT14)1
Former Common nameDsRed2hK14 Tg, C57BL/6, C57BL/6-Tg(hK5-DsRed2/hK14)1
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Koji Nishifuji, Keio University School of Medicine in 2006. This transgene contains the human keratin 5 promoter, a red fluorescent protein cDNA (DsRed2) that was fused to the N-terminal of human K14 gene in the basal lamina of stratified squamous epithelium.
Strain descriptionKeratin 14 is a intermediate filament mainly expressed in the basal cell of a stratified squamous epithelium. It is a component of the cytoskelton and combines with the intracellularly territory of the desmosome which is a cytoadherence machinery. This transgenic mouse shows the expression of a red fluorescent protein cDNA (DsRed2) that was fused to the N-terminal of human K14 gene under control of the human kratin 5 promoter in the basal lamina of stratified squamous epithelium. This mouse is useful for the study of the function of the cell adehision of the desmosome and the mechanism of the onset of autoimmune bullous disease.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DsRed2Red Fluorescent Protein (Discosoma sp.)UNDsRed2human Keratin 5 promoter
KRT14Keratin 14 (human)UNKRT14human Keratin 5 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAヒトケラチン 5プロモーター、イソギンチャクDsRed2 cDNA, ヒトケラチン 14 cDNA
    Research applicationDermatology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorMasayuki Amagai (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: TBUSAMTA Nonprofit
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data