Strain Data Sheet

RBRC02251

Strain Information

Image
BRC No.RBRC02251
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Nrp2<tm1Mkl>
Former Common nameneuropilin2 Knock out mouse
H-2 Haplotype
ES Cell lineCGR8 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Seiji Takashima, Osaka University Graduate School of Medicine in 1998. CGR8 ES cells were used.
Strain descriptionNeuropilin 2 gene knockout mice. Exon1 and part of the downstream intron including ATG was replaced with a lacZ-neo cassette. Neuropilin 2 expression was successfully stained with lacZ in various tissue using heterozygous mice. Homozygous mutant mice have vascular and neuronal abnormality and die in uterus or just after birth.
Colony maintenanceHetero mice are healthy and fertile. No specific care is needed. Homo mouse is dead in uterus or soon after birth.
References
Targeting of both mouse neuropilin-1 and neuropilin-2 genes severely impairs developmental yolk sac and embryonic angiogenesis.
Takashima S, Kitakaze M, Asakura M, Asanuma H, Sanada S, Tashiro F, Niwa H, Miyazaki Ji J, Hirota S, Kitamura Y, Kitsukawa T, Fujisawa H, Klagsbrun M, Hori M
Proc. Natl. Acad. Sci. USA, 99, 3657-3662 (2002). 11891274

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Nrp2neuropilin 21Nrp2targeted mutation 1, Michael Klagsbrun
lacZbeta-galactosidase (E. coli)1
neoneomycin resistance gene (E. coli)1

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal dorsal aorta morphology(MP:0004787)

  • abnormal nervous system development(MP:0003861)

  • abnormal neuronal migration(MP:0006009)

  • abnormal vascular development(MP:0000259)

  • abnormal vitelline vasculature morphology(MP:0003229)
    • more 5 phenotypes
  • decreased embryo size(MP:0001698)

  • embryo phenotype(MP:0005380)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)

  • hemorrhage(MP:0001914)

  • mortality/aging(MP:0010768)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli lacZ, E. coli neo, Mouse Neuropilin2 genomic DNA
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Hematological Research
    Neurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 3657-3662 (2002).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorSeiji Takashima (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data