RBRC02250

Information1

Image
BRC No.RBRC02250
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(CAG-Cox8/EGFP)49Rin
Former Common namemtGFP-Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Hiroshi Shitara and Hiromichi Yonekawa, Tokyo Metropolitan Institute of Medical Science in 2001. C57BL/6J background.
Strain descriptionThese transgenic express GFP in mitochondoria. This strain is useful for morphological analysis of mitochondoria in vivo. Tg hemizygous mice have a tendency to exhibit small body size, and females of hemizygote show lower reproductive performance. Homozygous mice are infertile.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesFEBS Letters, 500, 7-11, (2001). 11434917
Exp Anim. 2010;59(1):99-103 20224174

Health Report

Examination Date / Room / Rack2019/01/28Room:4-ARack:B
2018/11/26Room:4-ARack:B
2018/10/23Room:4-ARack:B
2018/10/23Room:4-ARack:B
2018/10/05Room:4-ARack:B
2018/09/25Room:4-ARack:B
2018/07/30Room:4-ARack:B
2018/05/21Room:4-ARack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Cox8acytochrome c oxidase, subunit VIIIaUNCox8aCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBB2rabbit beta-globin polyAUNHBB2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
MLSmitochondria localization signal (mouse cytochrome C)UNMLS

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mouse cytochrome C oxidase subunit VIII (mitochondrial inner membrane electron transport chain), HA1 epitope nucleotide sequence(synthetic), jellyfish enhancer green fluorescent protein (EGFP) cDNA
Research applicationCell Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Letters, 500, 7-11 (2001). Exp. Anim., 59, 99-103 (2010).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorHiromichi Yonekawa (Tokyo Metropolitan Institute of Medical Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

Esteves TC, Psathaki OE, Pfeiffer MJ, Balbach ST, Zeuschner D, Shitara H, Yonekawa H, Siatkowski M, Fuellen G, Boiani M.
Mitochondrial physiology and gene expression analyses reveal metabolic and translational dysregulation in oocyte-induced somatic nuclear reprogramming.
PLoS ONE 7(6) e36850(2012) 22693623