Strain Data Sheet
RBRC02250
Strain Information | |
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| Image |  |
| BRC No. | RBRC02250 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(CAG-Cox8/EGFP)49Rin |
| Former Common name | mtGFP-Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Hiroshi Shitara and Hiromichi Yonekawa, Tokyo Metropolitan Institute of Medical Science in 2001. C57BL/6J background. |
| Strain description | These transgenic express GFP in mitochondoria. This strain is useful for morphological analysis of mitochondoria in vivo. Tg hemizygous mice have a tendency to exhibit small body size, and females of hemizygote show lower reproductive performance. Homozygous mice are infertile. |
| Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
| References | Non-invasive visualization of sperm mitochondria behavior in transgenic mice with introduced green fluorescent protein (GFP). Shitara H, Kaneda H, Sato A, Iwasaki K, Hayashi J, Taya C, Yonekawa H FEBS Lett., 500, 7-11 (2001). 11434917Global imaging of mitochondrial morphology in tissues using transgenic mice expressing mitochondrially targeted enhanced green fluorescent protein. Shitara H, Shimanuki M, Hayashi J, Yonekawa H Exp Anim. 2010;59(1):99-103 20224174 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Cox8a | cytochrome c oxidase, subunit VIIIa | UN | Cox8a | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| GFP | Green Fluorescent Protein (Aequorea victoria) | UN | |||||
| HBB2 | rabbit beta-globin polyA | UN | HBB2 | ||||
| MLS | mitochondria localization signal (mouse cytochrome C) | UN | MLS | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), mouse cytochrome C oxidase subunit VIII (mitochondrial inner membrane electron transport chain), HA1 epitope nucleotide sequence(synthetic), jellyfish enhancer green fluorescent protein (EGFP) cDNA |
| Research application | Cell Biology Research Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Letters, 500, 7-11 (2001). Exp. Anim., 59, 99-103 (2010).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Hiromichi Yonekawa (Tokyo Metropolitan Institute of Medical Science) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Esteves TC, Psathaki OE, Pfeiffer MJ, Balbach ST, Zeuschner D, Shitara H, Yonekawa H, Siatkowski M, Fuellen G, Boiani M. Mitochondrial physiology and gene expression analyses reveal metabolic and translational dysregulation in oocyte-induced somatic nuclear reprogramming. PLoS One 7(6) e36850(2012) 22693623 |