Strain Data Sheet
RBRC02237
Information1 | |
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| Image |  |
| BRC No. | RBRC02237 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6Cr-Tg(Dmp1-HBEGF) |
| Former Common name | DTR-9.6 kb Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NCrSlc |
| Appearance | black [a/a B/B C/C] |
| Strain development | Developed by Drs. Tatsumi and Ikeda, National Center for Geriatrics and Gerontology in 2003. |
| Strain description | Bone remodeling consists of two distinct stages, resorption and formation, which are performed by osteoclasts and osteoblasts, respectively. Dmp1-HBEGF transgenic mice express human HB-EGF as the receptor for diphtheria toxin (DT) on the cell surface of osteocytes under the control of Dmp1 promoter. Most of the osteocytes (70 to 80%) can be ablated in these transgenic mice by injection of DT. The osteocytes-ablated mice develop osteoporosis, even in young age, showing fragile bone with intracortical porosity and microfractures, osteoblastic dysfunction, and loss of trabecular bone with microstructural deterioration and adipose tissue proliferation in the marrow space. Interestingly, the mice do not respond to mechanical unloading with bone loss. This strain is a useful model for the study of osteoporosis and mechanotransduction in bone. |
| Colony maintenance | Backcross to C57BL/6 (Hemizygote x C57BL/6CrSlc) |
| References | Cell Metab., 6, 464-475 (2007). 17550781 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBEGFheparin-binding EGF-like growth factor (human)UNHBEGFDmp1 (dentin matrix protein 1) promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signalUN |
Information2 | |
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| Donor DNA | mouse DMP1(dentin matrix protein 1) promoter, human HB-EGF cDNA, SV40 polyA signal |
| Research application | Mouse Models for Human Disease |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Metab., 6, 464-475, (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Kyoji Ikeda (National Center for Geriatrics and Gerontology) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Mouse of the Month Oct 2007 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Matsumoto T, Iimura T, Ogura K, Moriyama K, Yamaguchi A. The role of osteocytes in bone resorption during orthodontic tooth movement. J Dent Res 92(4) 340-5(2013) 23358677 Kwon RY, Meays DR, Meilan AS, Jones J, Miramontes R, Kardos N, Yeh JC, Frangos JA. Skeletal adaptation to intramedullary pressure-induced interstitial fluid flow is enhanced in mice subjected to targeted osteocyte ablation. PLoS One 7(3) e33336(2012) 22413015 |