RBRC02237

Information1

Image
BRC No.RBRC02237
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(Dmp1-HBEGF)
Former Common nameDTR-9.6 kb Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Tatsumi and Ikeda, National Center for Geriatrics and Gerontology in 2003.
Strain descriptionBone remodeling consists of two distinct stages, resorption and formation, which are performed by osteoclasts and osteoblasts, respectively. Dmp1-HBEGF transgenic mice express human HB-EGF as the receptor for diphtheria toxin (DT) on the cell surface of osteocytes under the control of Dmp1 promoter. Most of the osteocytes (70 to 80%) can be ablated in these transgenic mice by injection of DT. The osteocytes-ablated mice develop osteoporosis, even in young age, showing fragile bone with intracortical porosity and microfractures, osteoblastic dysfunction, and loss of trabecular bone with microstructural deterioration and adipose tissue proliferation in the marrow space. Interestingly, the mice do not respond to mechanical unloading with bone loss. This strain is a useful model for the study of osteoporosis and mechanotransduction in bone.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
ReferencesCell Metab., 6, 464-475, (2007). 17550781

Health Report

Examination Date / Room / Rack2017/01/16Room:2-検Rack:D
2016/11/14Room:2-検Rack:D
2009/02/23Room:3-CRack:D
2008/12/15Room:3-CRack:D
2008/10/20Room:3-CRack:D
2008/08/25Room:3-CRack:D
2008/06/23Room:3-CRack:D
2008/04/22Room:3-CRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
HBEGFheparin-binding EGF-like growth factor (human)UNHBEGFDmp1 (dentin matrix protein 1) promoter

Information2

Donor DNAmouse DMP1(dentin matrix protein 1) promoter, human HB-EGF cDNA, SV40 polyA signal
Research applicationMouse Models for Human Disease
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Metab., 6, 464-475, (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKyoji Ikeda (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Mouse of the Month Oct 2007

BRC mice in Publications

Matsumoto T, Iimura T, Ogura K, Moriyama K, Yamaguchi A.
The Role of Osteocytes in Bone Resorption during Orthodontic Tooth Movement.
J. Dent. Res. (2013) 23358677

Kwon RY, Meays DR, Meilan AS, Jones J, Miramontes R, Kardos N, Yeh JC, Frangos JA.
Skeletal adaptation to intramedullary pressure-induced interstitial fluid flow is enhanced in mice subjected to targeted osteocyte ablation.
PLoS ONE 7(3) e33336(2012) 22413015