Strain Data Sheet

RBRC02237

Strain Information

Image
BRC No.RBRC02237
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(Dmp1-HBEGF)
Former Common nameDTR-9.6 kb Tg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Tatsumi and Ikeda, National Center for Geriatrics and Gerontology in 2003.
Strain descriptionBone remodeling consists of two distinct stages, resorption and formation, which are performed by osteoclasts and osteoblasts, respectively. Dmp1-HBEGF transgenic mice express human HB-EGF as the receptor for diphtheria toxin (DT) on the cell surface of osteocytes under the control of Dmp1 promoter. Most of the osteocytes (70 to 80%) can be ablated in these transgenic mice by injection of DT. The osteocytes-ablated mice develop osteoporosis, even in young age, showing fragile bone with intracortical porosity and microfractures, osteoblastic dysfunction, and loss of trabecular bone with microstructural deterioration and adipose tissue proliferation in the marrow space. Interestingly, the mice do not respond to mechanical unloading with bone loss. This strain is a useful model for the study of osteoporosis and mechanotransduction in bone.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
References
Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction.
Tatsumi S, Ishii K, Amizuka N, Li M, Kobayashi T, Kohno K, Ito M, Takeshita S, Ikeda K
Cell Metab., 6, 464-475 (2007). 17550781

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
  • autosomal recessive hypophosphatemic rickets(DisGeNET)

  • hypophosphatemic rickets, autosomal recessive, 1(DisGeNET, MedGEN)
    • HBEGFheparin-binding EGF-like growth factor (human)UNHBEGFDmp1 (dentin matrix protein 1) promoter
  • autosomal recessive hypophosphatemic rickets(DisGeNET)

  • hypophosphatemic rickets, autosomal recessive, 1(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • decreased bone mass(MP:0004016)

  • osteoporosis(MP:0000066)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse DMP1(dentin matrix protein 1) promoter, human HB-EGF cDNA, SV40 polyA signal
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Metab., 6, 464-475, (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorKyoji Ikeda (National Center for Geriatrics and Gerontology)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Oct 2007

    BRC mice in Publications

    Matsumoto T, Iimura T, Ogura K, Moriyama K, Yamaguchi A.
    The role of osteocytes in bone resorption during orthodontic tooth movement.
    J Dent Res 92(4) 340-5(2013) 23358677
    Kwon RY, Meays DR, Meilan AS, Jones J, Miramontes R, Kardos N, Yeh JC, Frangos JA.
    Skeletal adaptation to intramedullary pressure-induced interstitial fluid flow is enhanced in mice subjected to targeted osteocyte ablation.
    PLoS One 7(3) e33336(2012) 22413015