Strain Data Sheet

RBRC02234

Strain Information

Image
BRC No.RBRC02234
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Calr<tm1Osb>
Former Common namecarl floxed mice
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masato Ikawa, Osaka University in 2005.
Strain descriptionCalreticulin, Calr is a major calcium binding protein located in the endoplasmic reticulum lumen. Conditional Calr deficient mice can be generated by crossing with tissue specific Cre transgenic mice.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc).Homozygous mutant mice are viable and fertile.
References
Calreticulin is required for development of the cumulus oocyte complex and female fertility.
Tokuhiro K, Satouh Y, Nozawa K, Isotani A, Fujihara Y, Hirashima Y, Matsumura H, Takumi K, Miyano T, Okabe M, Benham A M, Ikawa M
Sci. Rep., 2015 Sep 21;5:14254. doi: 10.1038/srep14254. 26388295

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Calrcalreticulin8Calrtargeted mutation 1, Research Institute for Microbial Diseases, Osaka University
  • myelofibrosis(MedGEN)

  • primary myelofibrosis(DisGeNET)

  • thrombocythemia 1(MedGEN)
    • Frtyeast FRT (flippase recombination target) site8Frt
  • myelofibrosis(MedGEN)

  • primary myelofibrosis(DisGeNET)

  • thrombocythemia 1(MedGEN)
    • loxPphage P1 loxP8loxP
  • myelofibrosis(MedGEN)

  • primary myelofibrosis(DisGeNET)

  • thrombocythemia 1(MedGEN)
    • loxPphage P1 loxP8loxP
  • myelofibrosis(MedGEN)

  • primary myelofibrosis(DisGeNET)

  • thrombocythemia 1(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal intercellular signaling peptide or protein level(MP:0008835)

  • abnormal oocyte morphology(MP:0001125)

  • abnormal tertiary ovarian follicle morphology(MP:0009363)

  • decreased litter size(MP:0001935)

  • decreased ovulation rate(MP:0003355)
    • more 3 phenotypes
  • embryonic lethality, complete penetrance(MP:0011092)

  • reduced female fertility(MP:0001923)

  • small ovary(MP:0001127)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Calr genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data