RBRC02234

Information1

Image
BRC No.RBRC02234
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Calr<tm1Osb>
Former Common namecarl floxed mice
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masato Ikawa, Osaka University in 2005.
Strain descriptionCalreticulin, Calr is a major calcium binding protein located in the endoplasmic reticulum lumen. Conditional Calr deficient mice can be generated by crossing with tissue specific Cre transgenic mice.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc).Homozygous mutant mice are viable and fertile.
ReferencesSci. Rep., 2015 Sep 21;5:14254. doi: 10.1038/srep14254. 26388295

Health Report

Examination Date / Room / Rack2007/12/17Room:3-CRack:I
2007/10/15Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Calrcalreticulin8Calr<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP8loxP

Information2

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Calr genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data