BRC No.RBRC02231
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Atg5<tm1Nmz>
Former Common name
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Masahiko Hatano and Takeshi Tokuhisa, Graduate School of Medicine, Chiba University, and Noboru Mizushima, National Institute for Basic Biology in 2003. The knockout construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionAtg5 knockout mice. Exons 1 and 2 of Atg5 were replaced with a neomycin resistance gene. Homozygous mutant mice die during the early neonatal period due to a lack of nutrients. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional mice (floxed allele) are also available: B6.129S-Atg5<tm1Myok> (RBRC02975).
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesNature, 432, 1032-1036 (2004) 15525940

Health Report

Examination Date / Room / Rack2020/03/30Room:4-ARack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Atg5autophagy-related 5 (yeast)10Atg5<tm1Nmz>targeted mutation 1, Noboru Mizushima

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)10herpes simplex virus thymidine kinase promoter (HSV tk promoter)


Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Atg5 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 432, 1032-1036 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorNoboru Mizushima (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-
Lab HP
Mouse of the Month Jul 2009
Genetic Background

BRC mice in Publications

Wu Y, Li Y, Zhang H, Huang Y, Zhao P, Tang Y, Qiu X, Ying Y, Li W, Ni S, Zhang M, Liu L, Xu Y, Zhuang Q, Luo Z, Benda C, Song H, Liu B, Lai L, Liu X, Tse HF, Bao X, Chan WY, A Esteban M, Qin B, Pei D.
Autophagy and mTORC1 regulate the stochastic phase of somatic cell reprogramming.
Nat. Cell Biol. (2015) 25985393