RBRC02228

Information1

Image
BRC No.RBRC02228
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameTOP-GAL
Former Common name
H-2 Haplotype
ES Cell line
Background strainICR
Appearancealbino [c/c]
Strain developmentDeveloped by Yumiko Saga, National Institute of Genetics in 2005. The transgene was injected into the pronuclei of (C57BL/6 x C3H)F2 fertilized eggs. ICR mixed background.
Strain descriptionTransgenic mice for visualizing Wnt signal pathway. Wnt proteins belong to a family of secreted and lipid-modified glycoprotein that can activate intracellular signaling pathways, such as the Wnt/planar cell polarity, the Wnt/calcium and the Wnt beta-catenin or so-called Wnt canonical signaling pathways. The TOP-GAL transgenic mice are a reporter line which can visualize the Wnt canonical pathway by beta-galactosidase activity. The transgenic mice were generated with ins-TOPGAL that accurately drive beta-galactosidase under TCF/beta-catenin. These transgenic mice will provide you unique opportunity to investigate the function of Wnt canonical pathway in vivo.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]. Tg mice are viable and fertile.
ReferencesGenesis, 45, 90-100 (2007) 17245775

Health Report

Examination Date / Room / Rack2014/08/04Room:3-1Rack:B
2014/06/02Room:3-1Rack:B
2014/05/12Room:3-1Rack:B
2014/03/10Room:2-検Rack:C
2014/01/14Room:2-検Rack:C
2013/11/11Room:2-検Rack:C
2013/09/09Room:2-検Rack:C
2013/07/24Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)UNTOPFLUSH promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

Donor DNATCF binding site from TOPFLUSH vector (Upstate) , HSV TK promoter, E. coli LacZ, SV40-late-poly A, chicken β-globin HS4 insulater, Simian virus 40 Large T antigen nuclear localization signal (nls)
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
General Purpose
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 45, 90-100 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Saga Lab HP
Genotyping protocol -PCR-
Mouse of the Month Aug 2009
Genetic Background

BRC mice in Publications

Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, Kominami R.
Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after -irradiation through Wnt/ -catenin pathway.
Carcinogenesis (2015) 25827435

Garbe DS, Ring RH.
Investigating Tonic Wnt Signaling Throughout the Adult CNS and in the Hippocampal Neurogenic Niche of BatGal and Ins-TopGal Mice.
Cell. Mol. Neurobiol. (2012) 22491991

Lu TL, Chen CM.
Differential requirements for beta-catenin in murine prostate cancer originating from basal versus luminal cells.
J. Pathol. 236(3) 290-301(2015) 25712462

Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y.
beta-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia.
Sci Rep 4 6959(2014) 25376241