Strain Data Sheet

RBRC02217

Strain Information

Image
BRC No.RBRC02217
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(MoMuLV/RET-MEN2A)121Mat
Former Common nameTg2A-121
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack (a/a B/? C/C)
brown (a/a b/b C/C)
Strain developmentDeveloped by Dr. K. Kawai, Nagoya University School of Medicine in 1999. C57BL/6 and BALB/c mixed background.
Strain descriptionThis transgenic mouse was generated by introducing RET with a MEN2A (cysteine 634 arginine) mutation fused to Moloney murine leukemia virus long terminal repeat. The heterozygous mice develop thyroid C-cell hyperplasia or medullary carcinoma.
Colony maintenanceSibling Mating (Heterozygote x Wild-type)
References
Tissue-specific carcinogenesis in transgenic mice expressing the RET proto-oncogene with a multiple endocrine neoplasia type 2A mutation.
Kawai K, Iwashita T, Murakami H, Hiraiwa N, Yoshiki A, Kusakabe M, Ono K, Iida K, Nakayama A, Takahashi M
Cancer Research, 60, 5254-5260 (2000). 11016655

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
RETrecombinant RET proto-oncogene (human)UNRETMoMuLV LTR
polyArat insulinUNpolyA

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal mitotic spindle morphology(MP:0009760)

  • cardiac interstitial fibrosis(MP:0005608)

  • enlarged thyroid gland(MP:0005355)

  • increased circulating calcium level(MP:0000194)

  • increased mammary adenocarcinoma incidence(MP:0001883)
    • more 3 phenotypes
  • increased metastatic potential(MP:0001272)

  • increased salivary adenocarcinoma incidence(MP:0000621)

  • increased thyroid C-cell carcinoma incidence(MP:0010345)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman c-Ret cDNA (MEN2A-type mutant), MoMuLV LTR, rat insulin poly A, SV40 poly(A) signal
    Research applicationCancer Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Researh, 60, 5254-5260 (2000).
    DepositorMasahide Takahashi (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data