Strain Data Sheet

RBRC02214

Strain Information

Image
BRC No.RBRC02214
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Gfra3<tm1Hmd>
Former Common nameGfra3 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
GFR alpha3, a component of the artemin receptor, is required for migration and survival of the superior cervical ganglion.
Nishino J, Mochida K, Ohfuji Y, Shimazaki T, Meno C, Ohishi S, Matsuda Y, Fujii H, Saijoh Y, Hamada H
Neuron, 23, 725-736 (1999). 10482239

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gfra3glial cell line derived neurotrophic factor family receptor alpha 318Gfra3targeted mutation 1, Hiroshi Hamada
neoneomycin resistance gene (E. coli)18SV40 and herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal neuronal migration(MP:0006009)

  • abnormal superior cervical ganglion morphology(MP:0001011)

  • abnormal sympathetic neuron innervation pattern(MP:0009284)

  • absent superior cervical ganglion(MP:0001014)

  • blepharoptosis(MP:0001344)
    • more 3 phenotypes
  • increased neuron apoptosis(MP:0003203)

  • small superior cervical ganglion(MP:0001015)

  • superior cervical ganglion degeneration(MP:0009561)
    • Detailed phenotype data

    Ordering Information

    Donor DNASV40 early genes promoter, HSV tk promoter, E. coli neo, SV40 Ter polyA addition signal
    Research applicationDevelopmental Biology Research
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 23, 725-736 (1999).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data