Strain Data Sheet

RBRC02212

Strain Information

Image
BRC No.RBRC02212
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Isl1-EGFP)1Okn
Former Common nameIsl-1GFP, zIsl-1GFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hideyuki Okano, Keio University, School of Medicine in 2000.
Strain descriptionThis transgenic mouse expresses GFP under the control of zebrafish Islet-1 promoter/enhancer, especially in the ventral motor neurons of the spinal cord. The transcription factor Islet-1 is expressed in the motor or sensory neurons of the spinal cord.
Colony maintenance
References
Comparative functional genomics revealed conservation and diversification of three enhancers of the isl1 gene for motor and sensory neuron-specific expression.
Uemura O, Okada Y, Ando H, Guedj M, Higashijima S, Shimazaki T, Chino N, Okano H, Okamoto H
Dev. Biol., 278, 587-606 (2005) 15680372

Visualization of cranial motor neurons in live transgenic zebrafish expressing green fluorescent protein under the control of the islet-1 promoter/enhancer.
Higashijima S, Hotta Y, Okamoto H
J. Neurosci. 20, 206-218 (2000) 10627598

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFPGreen Fluorescent Protein (Aequorea victoria)UNzebrafish islet-1 promoter/enhancer

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAzebrafish islet-1 promoter, Jellyfish EGFP cDNA, SV40 intron pA, pBluescript
    Research applicationNeurobiology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorHideyuki Okano (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data