Strain Data Sheet

RBRC02192

Information1

Image
BRC No.RBRC02192
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mapk14<tm1.2Otsu>
Former Common namep38α-flox mouse
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Kinya Otsu, Osaka University Graduate School of Medicine in 2000. The targeting construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionMitogen-activated protein (MAP) kinase cascades are highly conserved signal transduction pathways involved in a variety of physiological events. The MAP kinase family consists of extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal protein kinase (JNK), and p38 MAP kinase. p38 alpha, one of the p38 MAP kinase subfamily, is widely expressed and has an important function in cytokine production and the response to many types of stress. Because p38alpha-deficient mice (BRC00361 C57BL/6-TgH(p38)/118) showed embryonic lethality at midgestation, this strain is useful for the research of p38 MAP kinase pathway in various tissues using Cre-loxP system.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
ReferencesMol. Cell. Biol., 24, 10611-10620 (2004). 15572667

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mapk14mitogen activated protein kinase 1417Mapk14<tm1.2Otsu>targeted mutation 1.2, Kinya OtsuCrk1, Csbp1, CSBP2, Mxi2, p38, p38 MAP Kinase, p38-alpha, p38MAPK, tRNA synthetase cofactor p38

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Information2

Donor DNAPhage P1 loxP sites, mouse p38 alpha genomic DNA
Research applicationCell Biology Research
Cre/loxP system
General Purpose
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 24, 10611-10620 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKinya Otsu (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Jul 2007
Genetic Background
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

Krementsov DN, Noubade R, Dragon JA, Otsu K, Rincon M, Teuscher C.
Sex-specific control of central nervous system autoimmunity by p38 mitogen-activated protein kinase signaling in myeloid cells.
Ann Neurol 75(1) 50-66(2014) 24027119

Schnöder L, Tomic I, Schwindt L, Helm D, Rettel M, Schulz-Schaeffer W, Krause E, Rettig J, Fassbender K, Liu Y.
P38α-MAPK phosphorylates Snapin and reduces Snapin-mediated BACE1 transportation in APP-transgenic mice.
FASEB J 35(7) e21691(2021) 34118085

Schnöder L, Gasparoni G, Nordström K, Schottek A, Tomic I, Christmann A, Schäfer KH, Menger MD, Walter J, Fassbender K, Liu Y.
Neuronal deficiency of p38α-MAPK ameliorates symptoms and pathology of APP or Tau-transgenic Alzheimer's mouse models.
FASEB J 34(7) 9628-9649(2020) 32475008

Schnöder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y.
Deficiency of Neuronal p38α MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1.
J Biol Chem 291(5) 2067-79(2016) 26663083

Liu X, Ma B, Malik AB, Tang H, Yang T, Sun B, Wang G, Minshall RD, Li Y, Zhao Y, Ye RD, Xu J.
Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases.
Nat Immunol 13(5) 457-64(2012) 22447027

Hutchison MR.
Mice with a conditional deletion of the neurotrophin receptor TrkB are dwarfed, and are similar to mice with a MAPK14 deletion.
PLoS One 8(6) e66206(2013) 23776632

Kase Y, Otsu K, Shimazaki T, Okano H.
Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling.
Stem Cell Reports 12(6) 1313-1328(2019) 31080114