RBRC02190

Information1

Image
BRC No.RBRC02190
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.129P2-Nfe2l2<tm1Mym>
Former Common nameBALB/c(F10)-nfe2l2<tm1Mym>
H-2 Haplotype
ES Cell line
Background strainBALB/cAJcl
Appearancealbino [A/A b/b c/c]
Strain developmentDeveloped by Masayuki Yamamoto, Institute of Basic Medical Sciences and Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 1996. The targeting vector containing lacZ-neo cassette was transferred into E14 ES cells to replace the 1.2 kb segment containing the rest of the exon 5 coding sequence of the Nrf2 gene.
Strain descriptionNrf2 (Nfe2l2) Knockout mice. This strain is a useful model for the in vivo analysis of chemical carcinogenesis and resistance to anti-cancer drugs. A phenolic antioxidant significantly induced the expression of phase II enzymes such as glutathione S-transferase and NAD(P)H: quinone oxidoreductase in normal mice. However, in the homozygous Nrf2 deficient mice the induction of the phase II enzymes by a phenolic antioxidant was largely eliminated in the liver and intestine. Since Nrf2 is constantly degraded by proteasome under normal condition, it is hardly to detect Nrf2 protein in the absence of stimuli. Electrophilic chemicals, such as DEM (diethyl maleate) and tBHQ (tert-buthyl hydroquinone), are generally used to stabilize Nrf2. Or proteasomal inhibitors, such as MG132, are also effective increasing the protein amount of Nrf2. C57BL/6 background (RBRC01390), BALB/c background (RBRC02190), A/J background (RBRC02414), ICR mixed background (RBRC00984). Homozygous mutant mice are viable and fertile, but show lower reproductive performance.
Colony maintenanceHomozygote x Heterozygote [or Crossing to BALB/cAJcl]
ReferencesBiochem. Biophys. Res. Commun., 236, 313-322 (1997). 9240432

Health Report

Examination Date / Room / Rack2010/09/13Room:4-BRack:F
2010/07/12Room:4-BRack:F
2010/05/17Room:4-BRack:F
2010/03/15Room:4-BRack:F
2010/01/18Room:4-BRack:F
2009/07/13Room:4-BRack:F
2009/05/19Room:4-BRack:F
2009/05/18Room:4-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nfe2l2nuclear factor, erythroid derived 2, like 22Nfe2l2<tm1Mym>targeted mutation 1, Masayuki Yamamoto

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)2herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)2nls

Information2

Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Nrf2 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun. 236,313-322 (1997).The DEPOSITEOR should be a co-author in the articles when the RECIPIENT publish their data using these mice for 2 year after deposition to the RIKEN BRC. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from these mice.
DepositorMasayuki Yamamoto (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Lab HP (Japanese)
Genotyping protocol -PCR-
Mouse of the Month Apr 2012

BRC mice in Publications

No Data