RBRC02189

Information1

Image
BRC No.RBRC02189
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(Pcdh21-cre)BYoko
Former Common namePcdh21-nCre, C57BL/6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi Kyoto University Guraduate School of Medicine nin 2004.
Strain descriptionThis transgenic mice express the cre recombinase gene under the control of the Pcdh21 gene promoter. This can be used to make an conditional knock-out by crossing with the other genetically-engineered mice that have floxed genes. The expression of cre recombinase was observed in the mitral/tufted cells that are projection neurons in the olfactory bulb.
Colony maintenanceBackcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
ReferencesGenesis, 43, 12-16 (2005). 16106355

Health Report

Examination Date / Room / Rack2012/04/16Room:3-CRack:D
2012/02/20Room:3-CRack:D
2011/12/12Room:3-CRack:D
2011/10/26Room:3-CRack:D
2011/10/25Room:3-CRack:D
2011/10/20Room:3-CRack:D
2011/10/17Room:3-CRack:D
2011/08/29Room:3-CRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinaseUNcrePcdh21 promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAPcdh21 promoter

Information2

Donor DNAFLAG tag, tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), human FGF2-IRES, nuclear localization signal, Phage P1 Cre recombinase, rabbit beta-globin intron/polyA signal, mouse pcdh21 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 43, 12-16 (2005).
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Genetic Background

BRC mice in Publications

Miller JE, Granados-Fuentes D, Wang T, Marpegan L, Holy TE, Herzog ED.
Vasoactive intestinal polypeptide mediates circadian rhythms in Mammalian olfactory bulb and olfaction.
J. Neurosci. 34(17) 1510796(2014) 24760863

Iwata R, Kiyonari H, Imai T.
Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb.
Neuron 96(5) 1139-1152.e7(2017) 29216451