Strain Data Sheet

RBRC02188

Strain Information

Image
BRC No.RBRC02188
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(tetO-APTTC)1Yoko
Former Common nameB6.tetO-APTTC,C57BL/6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Kyoto University Graduate School of Medicine in 2004. The transgene was injected into the pronuclei of C57BL/6CrSlc fertilized eggs.
Strain descriptionThis transgenic mice express a fusion gene, APTTC, consisting of the truncated human placental alkaline phosphatase with TTC (Tetanus toxin C fragment). This strain can be used to make an inducible expression of APTTC as a retrograde transynaptic genetic tracer corssing with the genetically-engineered mice that have a tetracycline-controlled transactivator (tTA) in any specific neurons.
Colony maintenanceCarrier x Noncarrier [C57BL/6CrSlc]Tg/+ hemizygous mice are viable and fertile.
References
Inducible expression of retrograde transynaptic genetic tracer in mice.
Sano H, Nagai Y, Yokoi M
Genesis, 45, 123-128 (2007). 17299748

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signalUN
PLAPhuman placental alkaline phosphataseUNPLAPE. coli tetO (tetracycline operator)
TTCnon-toxic c fragment (Clostridium tetani)UNTTC

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli tetracycline operator(tetO) promoter, artificial intron, splice site, human placental alkaline phosphatase cDNA, Clostridium tetani non-toxic c fragment, SV40 polyA signal
    Research applicationNeurobiology Research
    Tet system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 45, 123-128 (2007).
    DepositorYokoi Mineto (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data