RBRC02188

Information1

Image
BRC No.RBRC02188
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6Cr-Tg(tetO-APTTC)1Yoko
Former Common nameB6.tetO-APTTC,C57BL/6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Mineto Yokoi, Kyoto University Graduate School of Medicine in 2004. The transgene was injected into the pronuclei of C57BL/6CrSlc fertilized eggs.
Strain descriptionThis transgenic mice express a fusion gene, APTTC, consisting of the truncated human placental alkaline phosphatase with TTC (Tetanus toxin C fragment). This strain can be used to make an inducible expression of APTTC as a retrograde transynaptic genetic tracer corssing with the genetically-engineered mice that have a tetracycline-controlled transactivator (tTA) in any specific neurons.
Colony maintenanceCarrier x Noncarrier [C57BL/6CrSlc]Tg/+ hemizygous mice are viable and fertile.
ReferencesGenesis, 45, 123-128 (2007). 17299748

Health Report

Examination Date / Room / Rack2007/08/20Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
PLAPhuman placental alkaline phosphataseUNPLAPE. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TTCnon-toxic c fragment (Clostridium tetani)UNTTC

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signalUN

Information2

Donor DNAE. coli tetracycline operator(tetO) promoter, artificial intron, splice site, human placental alkaline phosphatase cDNA, Clostridium tetani non-toxic c fragment, SV40 polyA signal
Research applicationNeurobiology Research
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 45, 123-128 (2007).
DepositorYokoi Mineto (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data