Strain Data Sheet

RBRC02176

Strain Information

Image
BRC No.RBRC02176
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Clgn<tm1Osb>
Former Common nameB6-Clgn KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Masato Ikawa, Research Institute for Microbial Diseases, Osaka University in 1995. D3 ES cells derived from 129S2/SvPas were used. The mutant mice were backcrossed to C57BL/6.
Strain descriptionClgn, calmegin gene knockout mice. A neomycin selection cassette was inserted into the exon 2 of the Clgn gene. Homozygous mutant males were infertile.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrSlc]
References
The putative chaperone calmegin is required for sperm fertility.
Ikawa M, Wada I, Kominami K, Watanabe D, Toshimori K, Nishimune Y, Okabe M
Nature, 387, 607-611 (1997) 9177349

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Clgncalmegin8Clgntargeted mutation 1, Research Institute for Microbial Diseases, Osaka University
neoneomycin resistance gene (E. coli)8mouse phosphoglycerate kinase promoter (PGK promoter)
poly Amouse PGK polyA8poly A

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • impaired fertilization(MP:0000242)

  • male infertility(MP:0001925)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPGK promoter + neo<r> cDNA + PGK pA signal
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 387, 607-611 (1997).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data