Strain Data Sheet

RBRC02149

Strain Information

Image
BRC No.RBRC02149
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ctnnbip1<tm1Taki>
Former Common nameICAT Knock-out mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Akiyama at Institute for Molecular and Cellular Bioscviences, University of Tokyo in 2001.
Strain descriptionICAT, Inhibitor of beta-catenin and Tcell factor is an 81 amino acid protein that interferes with the interaction between beta-catenin and TCF. The ICAT gene ubiquitously expressed in embryo, and brain, heart, liver and skeletal muscle in adult of mouse. ICAT homozygous mutant mice show malformation of the forebrain and craniofacial bones and lack kidney, and dead within 1 day after birth.
Colony maintenanceHeterozygote x C57BL/6 [C57BL/6NJcl]
References
Anteriorization of neural fate by inhibitor of beta-catenin and T cell factor (ICAT), a negative regulator of Wnt signaling.
Satoh K, Kasai M, Ishidao T, Tago K, Ohwada S, Hasegawa Y, Senda T, Takada S, Nada S, Nakamura T, Akiyama T
Proc. Natl. Acad. Sci. USA, 101, 8017-8021 (2004). 15148409

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ctnnbip1catenin beta interacting protein 14Ctnnbip1targeted mutation 1, Tetsu Akiyama
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal apoptosis(MP:0001648)

  • abnormal cranium morphology(MP:0000438)

  • abnormal digestive system morphology(MP:0000462)

  • abnormal head morphology(MP:0000432)

  • abnormal metanephros morphology(MP:0011364)
    • more 15 phenotypes
  • abnormal nose morphology(MP:0002233)

  • abnormal rostral-caudal axis patterning(MP:0005221)

  • abnormal telencephalon development(MP:0000934)

  • abnormal ureteric bud invasion(MP:0010983)

  • absent kidney(MP:0000520)

  • absent maxilla(MP:0004539)

  • absent ureter(MP:0003722)

  • anophthalmia(MP:0001293)

  • cleft secondary palate(MP:0009890)

  • delayed kidney development(MP:0000528)

  • exencephaly(MP:0000914)

  • impaired branching involved in ureteric bud morphogenesis(MP:0004936)

  • increased metanephric mesenchyme apoptosis(MP:0011386)

  • neonatal lethality, complete penetrance(MP:0011087)

  • single kidney(MP:0003604)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase-1 promoter, E. coli neo, mouse ICAT genomic DNA
    Research applicationDevelopmental Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 101, 8017-8021 (2004).The RECIPIENT of BIOLOGICAL RESOURCE shall inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE, and agrees to include the DEPOSITOR as a co-author in any publications resulted by the used of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. After the two years' period the RECIPIENT shall acknowledge to the DEPOSITOR in any publications resulted by the use of the BIOLOGICAL RESOURCE.
    DepositorTetsu Akiyama (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data