RBRC02142

Information1

Image
BRC No.RBRC02142
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.129S2-Pdcd1<tm1Hon>/HonRbrc
Former Common nameC57BL/6-PD-1KO, C57BL/6-Pdcd1-/- mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strainC57BL/6
Appearanceblack [(a/a B/B C/C)]
Strain developmentDeveloped by Tasuku Honjo, Graduate School of Medicine, Kyoto University in 1998. The knockout construct was electoroporated into D3 ES cells derived from 129S2/SvPas. The mutant mice were backcrossed to C57BL/6 over 11 generations.
Strain descriptionPD1 knockout mice. EcoRV site of exon 3 to PvuII site of exon 5 was replaced with noemycin selection cassette. PD1 (Pdcd1, programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, plays a role in the negative control of proliferation, differentiation and class switching of B cells. PD1 deficiency has been shown to develop different forms of autoimmune diseases on different backgrounds of mice. Homozygous PD1 mutant mice of C57BL/6 background show moderate but consistent splenomegaly, and spontaneously develop characteristic lupus-like proliferative arthritis and golmerulonephritis with the age.
Colony maintenanceHomozygote x Homozygote.
ReferencesInt. Immunol.,10,1563-1572 (1998). 9796923
Immunity, 11, 141-151 (1999). 10485649
Science, 291, 319-322 (2001). 11209085

Health Report

Examination Date / Room / Rack2020/02/03Room:3-3Rack:A
2019/12/02Room:3-3Rack:A
2019/10/16Room:3-3Rack:A
2019/08/05Room:3-3Rack:A
2019/06/03Room:3-3Rack:A
2019/04/01Room:3-3Rack:A
2019/02/04Room:3-3Rack:A
2018/12/03Room:3-3Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pdcd1programmed cell death 11Pdcd1<tm1Hon>targeted mutation 1, Tasuku Honjo

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PD-1(immunoglobulin superfamily member) genomic DNA
Research applicationImmunology and Inflammation Research
Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
DepositorTasuku Honjo (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Honjo Lab HP
Genotyping protocol -PCR-
Mouse of the Month Jul 2012
Genetic Background
Breeding characters

BRC mice in Publications

Sabine Mumprecht, Christian Sch rch, Juerg Schwaller, Max Solenthaler, and Adrian F. Ochsenbein.
Programmed death 1 signaling on chronic myeloid leukemia-specific T cells results in T-cell exhaustion and disease progression.
Blood 114(8) 1528-36(2009) 19420358

Horne-Debets JM, Faleiro R, Karunarathne DS, Liu XQ, Lineburg KE, Poh CM, Grotenbreg GM, Hill GR, Macdonald KP, Good MF, Renia L, Ahmed R, Sharpe AH, Wykes MN.
PD-1 Dependent Exhaustion of CD8<sup>+</sup> T Cells Drives Chronic Malaria.
Cell Rep (2013) 24316071

Yokosuka T, Takamatsu M, Kobayashi-Imanishi W, Hashimoto-Tane A, Azuma M, Saito T.
Programmed cell death 1 forms negative costimulatory microclusters that directly inhibit T cell receptor signaling by recruiting phosphatase SHP2.
J. Exp. Med. (2012) 22641383

Miyajima M, Zhang B, Sugiura Y, Sonomura K, Guerrini MM, Tsutsui Y, Maruya M, Vogelzang A, Chamoto K, Honda K, Hikida T, Ito S, Qin H, Sanuki R, Suzuki K, Furukawa T, Ishihama Y, Matsuda F, Suematsu M, Honjo T, Fagarasan S.
Metabolic shift induced by systemic activation of T cells in PD-1-deficient mice perturbs brain monoamines and emotional behavior.
Nat. Immunol. 18(12) 1342-1352(2017) 29058703

Kawamoto S, Tran TH, Maruya M, Suzuki K, Doi Y, Tsutsui Y, Kato LM, Fagarasan S.
The inhibitory receptor PD-1 regulates IgA selection and bacterial composition in the gut.
Science 336(6080) 485-9(2012) 22539724